临床肝胆病杂志2017，Vol.33Issue(3)：462-465,4.DOI:10.3969/j.issn.1001-5256.2017.03.013
KIR-HLA基因多态性与散发性急性戊型肝炎的关系
Association between killer cell immunoglobulin-like receptor/human leukocyte antigen gene polymorphisms and sporadic acute hepatitis E
摘要
Abstract
Objective To investigate the association between killer cell immunoglobulin-like receptor (KIR)/human leukocyte antigen (HLA) gene polymorphisms and sporadic acute hepatitis E (AHE).Methods A total of 42 AHE patients who were hospitalized in First Department of Hepatitis,Public Health Clinical Center Affiliated to Fudan University,from August 2015 to September 2016 were enrolled,and 30 healthy subjects were enrolled as control group.Genomic DNA was extracted from the peripheral blood,and PCR/sequence specific primer was used for the amplification of KIR gene.Sanger sequencing was used to determine the genotype of HLA,and the association between KIR-HLA distribution and sporadic AHE was analyzed.The chi-square test was used for comparison of categorical data between groups.Results A total of 16 KIR genes were detected in each group,including inhibitory receptor genes 2DL1-3,2DL5,and 3DL1-3,activating receptor genes 2DS1-5 and 3DS1,the special inhibitory/activating receptor gene 2DI4,internal reference gene DRB1,and pseudogene 2DP1,as well as HLA-B (including HLA-Bw4,HLA-Bw6,and HLA-Bw4/Bw6) and HLA-C (including HLA-C1/C1,HLA-C1/C2,and HLA-C2/C2),were also detected.Compared with the control group,the AHE group had significantly lower frequencies of inhibitory genes KIR 2DL3 (64.3％ vs 93.3％,P ＜ 0.05),2DL5 (23.8％ vs 56.7％,P ＜ 0.05),3DL2 (71.4％ vs 100％,P ＜ 0.05),and 3 DL3 (69.0％ vs 100％,P ＜ 0.05),significantly lower frequencies of HLA-C1 C2 (19.0％ vs 40.0％,P ＜ 0.05) and 3DL1/HLA-Bw4 (45.2％ vs 73.3％,P ＜ 0.05),and a significantly higher frequency of HLA-C1/C1 gene (71.4％ vs 46.7％,P ＜0.05).Conclusion The difference in the distribution of inhibitory genes KIR 2DL3,2DL5,3DL2,and 3DL3,HLA-C1C1/C1C2,and 3DL1/HLA-Bw4 may be associated with sporadic AHE caused by hepatitis E virus infection.关键词
肝炎,戊型/多态性,单核苷酸/受体,自然杀伤细胞/HLA抗原/基因型Key words
hepatitis E/polymorphism, single nucleotide/receptors, natural killer cell/HLA antigens/genotype分类
医药卫生引用本文复制引用
陈冲,章树业,荀静娜,张玉玲,李微霞,卢川,陈良..KIR-HLA基因多态性与散发性急性戊型肝炎的关系[J].临床肝胆病杂志,2017,33(3):462-465,4.基金项目
国家重点基础研究发展计划“973计划”(2015CB554303) （2015CB554303）
上海申康医院发展中心市级医院新兴前沿技术联合攻关项目(SHDC12015129) （SHDC12015129）
上海市科委“科技创新行动计划”医学与农业领域重点项目(13401902100) （13401902100）
