环境与职业医学2017，Vol.34Issue(4)：330-336,7.DOI:10.13213/j.cnki.jeom.2017.16756
氟化钠对小鼠成骨细胞MC3T3-E1凋亡的影响
Effect of sodium fluoride on apoptosis of mice osteoblasts MC3T3-E1 cells
摘要
Abstract
[Objective] To test the effects of sodium fluoride (NaF) on apoptosis of osteoblasts MC3T3-E1.[Methods] MC3T3-E1 cells were exposed to different doses of NaF for 24h and 48 h,respectively.Cell viability was measured by CCK-8 to determine exposure concentrations and time for subsequent experiment.The osteoblasts were treated with 0,1 × 103,2 × 103,and 3 × 103 μmol/L NaF for 24 h and 48 h,respectively.Apoptosis was detected by flow cytometry.The expressions of Caspase-3 and Caspase-8 mRNA were detected by real-time PCR and the expression of Cleaved Caspase-3 and Cleaved PARP proteins were detected by Western blot.[Results] The results of CCK-8 assay showed that the survival rate of MC3T3-E1 cells decreased with the increase of NaF concentration and the prolongation of exposure time.At 1 × 103 μmol/L,the OD value of CCK-8 assay was lower than that of the control group,and the survival rate decreased (P ＜ 0.05).The O D value at 1 × 103 μmol/L for 48 h was also lower than that for 24 h (P ＜ 0.05).Compared with the control group,the apoptosis rates at different stages at 24h of the NaF treatment groups were significantly higher than that of the control group (P＜0.05),except the apoptosis rate at middle and late stages of the 1 × 103 μmol/L NaF group.The expression levels of Caspase-3 and Caspase-8 mRNA showed an increasing trend with the increasing concentration of NaF;and the expression levels of Cleaved Caspase-3 and Cleaved PARP also increased in all NaF groups (P＜0.05),except the expression of Cleaved PARP protein of the 1 × 103μmol/L NaF group.[Conclusion] The signal transduction mediated by Caspase plays an important role in the apoptosis induced by high concentration of sodium fluoride.关键词
氟化钠/MC3T3-E1细胞/细胞凋亡/Caspase-3/Caspase-8Key words
sodium fluoride/MC3T3-E1 cell/cell apoptosis/Caspase-3/Caspase-8分类
医药卫生引用本文复制引用
王楠兰,雷露,敖全,陈密,吴彦秋,张华,韦艳..氟化钠对小鼠成骨细胞MC3T3-E1凋亡的影响[J].环境与职业医学,2017,34(4):330-336,7.基金项目
国家自然科学基金(编号:81560515) （编号:81560515）
贵州省科技厅社会发展攻关计划项目(编号:黔科合SY字[2013]3062号) （编号:黔科合SY字[2013]3062号）
科技部国家科技支撑计划课题(编号:2013BAI05B03) （编号:2013BAI05B03）
