农业环境科学学报2017，Vol.36Issue(4)：635-642,8.DOI:10.11654/jaes.2016-1467
Cd胁迫诱导拟南芥幼苗DNA损伤分析
Analysis of Cd-induced DNA damage in Arabidopsis seedlings
摘要
Abstract
DNA damage assay based on RAPD, ELISA-based global methylation analysis, and expression analysis of genes related to DNA damage, repair, and cell cycle were used to study genomic DNA damage and stress response of DNA repair and cell cycle in Arabidopsis plantlets exposed to 0,0.125,0.25,1.0 mg·L-1 and 2.5 mg·L-1 cadmium(Cd)for 5 d. Compared with the control, DNA damage and global methylation rate were increased significantly with the increased Cd dose , and expression of cell division genes(PCNA 1 and PCNA 2), MMR genes(MLH1, MSH2 and MSH6), homologous recombination genes(RAD51 and BRCA1), and non- homologous end joining genes(KU70 , MRE11 and GR1)were followed an obvious inverted U- shaped dose- response effect of Cd exposures and a MMR>HR>NHEJ sensitivity rule. The results showed that low-dose Cd result in easy-repaired mismatch damage, and high-dose Cd stress cause diffi-cult-repaired chromosome damage and double strand break, which could accumulate and aggravate with the stress duration. In addition, the expression of MSH6 and MLH1 is the most sensitive indicator which could be a sensitive biomarker, applied to early diagnosis and risk as-sessment of genotoxic effects of Cd pollution in ecotoxicology.关键词
Cd/DNA损伤修复/DNA修复/细胞周期Key words
Cd/DNA damage/DNA repair/cell cycle分类
资源环境引用本文复制引用
宋婕,台培东,杨悦锁,刘宛,王鹤潼,崔伟娜,孙梨宗,曹霞,何蕾,姜丽思,成智博,惠秀娟..Cd胁迫诱导拟南芥幼苗DNA损伤分析[J].农业环境科学学报,2017,36(4):635-642,8.基金项目
国家自然科学基金项目(21677151,41673232,41472237) （21677151,41673232,41472237）
国家重点研发计划课题(2016YFD0800305) The National Natural Science Foundation of China(21677151,41673232,41472237) （2016YFD0800305）
The National Science and Technology Major Project of the Ministry of Science and Technology of China(2016YFD0800305) （2016YFD0800305）
