农药学学报2017，Vol.19Issue(2)：176-181,6.DOI:10.16801/j.issn.1008-7303.2017.0023
高效氯氰菊酯对草地贪夜蛾Sf9细胞自噬的诱导作用
Beta-cypermethrin induced Spodoptera frugiperda Sf9 cells autophagy research
摘要
Abstract
The cell viability and autophagic vacuoles of the beta-cypermethrin treated Sf9 cells (Spodoptera frugiperda cell) were evaluated and the autophagic-related proteins of Sf9 cells was analyzed. 3-(4,5-Dimethyl-2-thiazolyl)-2, 5-diphenyl-2-H-tetrazolium bromide(MTT) assay, microscope assay, transmission electron microscope(TEM) assay, monodansylcadaverine(MDC) fluorescent staining assay and western blot(WB) assay were employed for the investigation. Results showed that beta-cypermethrin inhibited the viability of Sf9 cells in both concentration- and time-dependent manners, and the autophagy was observed in beta-cypermethrin-treated cells. Autophagy-related protein LC3-Ⅱ/LC3-Ⅰand Beclin 1 were up-regulated to 201.32% and 136.00% compared to the control group. And protein p62 was down-regulated to 68.13%. It has been demonstrated that beta-cypermethrin has significant cytotoxicity on Sf9 cells and inhibition the cell's ability via the autophagy pathway.关键词
高效氯氰菊酯/草地贪夜蛾/Sf9细胞/细胞毒性/细胞自噬Key words
beta-cypermethrin/Spodoptera frugiperda/Sf9 cells/cytotoxic effects/cell autophagy分类
农业科技引用本文复制引用
杨韵,卢淼青,张阳,杨明俊,徐文平,陶黎明..高效氯氰菊酯对草地贪夜蛾Sf9细胞自噬的诱导作用[J].农药学学报,2017,19(2):176-181,6.基金项目
"十二五"国家科技支撑计划项目(2011BAE06B04). （2011BAE06B04）
