农药学学报2017，Vol.19Issue(2)：195-202,8.DOI:10.16801/j.issn.1008-7303.2017.0026
蓝莓枝枯病拮抗细菌HMQAU140045的鉴定和抑真菌活性
Identification and antifungal activity of antagonistic bacterium HMQAU140045 against blueberry twig blight
摘要
Abstract
Twenty strains of bacteria were isolated by the plate dilution method from the rhizosphere soil collected from the blueberry plantations in Qingdao, Shandong. Among them, a strain, HMQAU140045, with obvious inhibition effect on the target fungus, blueberry Lasiodiplodia twig blight pathogen- Lasiodiplodia pseudotheobromae, was discovered by the plate confrontation method and mycelial growth rate method. With the concave slide method and mycelial growth rate method, the effects of HMQAU140045 fermentation filtrate on the spore germination and mycelial growth of the target fungus were measured. The results showed that the inhibition rate of the 50 times diluted fermentation filtrate could reach 100%. The result of the detached twig experiment indicated that the control efficacies of different components against blueberry Lasiodiplodia twig blight in the fermentation broth are all above 90%. Bioassay results obtained using the mycelial growth rate method demonstrated that the strain was of broad antifungal spectrum, with obvious antifungal activity against fifteen plant pathogenic fungi, including four blueberry twig blight pathogens. Based on the morphological observation, physiological and biochemical characteristics, and the sequence analysis of gyrB, strain HMQAU140045 was identified as Bacillus amyloliquefaciens. These experimental results demonstrated that the strain HMQAU140045 of Bacillus amyloliquefaciens had good antifungal activity.关键词
解淀粉芽孢杆菌/蓝莓枝枯病/生物测定/促旋酶(gyrase)B亚单位基因/发酵液Key words
Bacillus amyloliquefaciens/blueberry twig blight/bioassay/gyrB/fermentation broth分类
农业科技引用本文复制引用
宫燕伟,王佳宁,梁晨,赵洪海..蓝莓枝枯病拮抗细菌HMQAU140045的鉴定和抑真菌活性[J].农药学学报,2017,19(2):195-202,8.基金项目
青岛市科技局项目(16-6-2-36-nsh). （16-6-2-36-nsh）
