农药学学报2017，Vol.19Issue(2)：245-253,9.DOI:10.16801/j.issn.1008-7303.2017.0033
液体农药制剂中烷基酚及其聚氧乙烯醚类助剂的高效液相色谱分析
Determination of alkylphenols and their ethoxylates in liquid pesticide formulations by high-performance liquid chromatography
摘要
Abstract
A simple method for simultaneous determination of alkylphenols (APs) and alkylphenol ethoxylates (APEOs) in six kinds of liquid pesticide formulations was developed using high-performance liquid chromatography with fluorescence detection (HPLC-FLD). Six kinds of liquid pesticide formulation samples (emulsifiable concentrate, aqueous solutions, emulsion in water, suspension concentrate, soluble concentrate, and microemulsion) were ultrasonically extracted with methanol. The extract was diluted with methanol and centrifuged. The supernatant was filtered through a polypropylene (PP) filter and then separated on an Agilent Poroshell C18 (4.6 mm × 100 mm, 2.7 μm) chromatographic column using an isocratic elution program of V (methanol):V (acetonitrile):V (water) =75.5 : 6.5 : 18.0. Qualitative analysis was performed by HPLC-FLD, and the external standard method was used to quantify the concentrations of target analytes. The results showed that good linearity between the peak areas of APs and APEOs and their mass concentrations (R2>0.9998) was obtained in the range of 0.05–10 and 0.1–20 mg/L. At three or four spiked levels (APs: 0.5, 2, 5 mg/g; APEOs: 1, 5, 25, 50 mg/g), the mean recoveries ranged from 73% to 110%, with relative standard deviations (RSD, n= 5) of 0.6%–14.9% in those six kinds of liquid pesticide formulations. The limits of quantitation (LOQ) varied from 0.5 mg/g to 1 mg/g. This new method is convenient, rapid, accurate and suitable for the simultaneous determination of APs and APEOs in those six kinds of liquid pesticide formulations.关键词
农药助剂/液体农药制剂/烷基酚/烷基酚聚氧乙烯醚/高效液相色谱Key words
pesticide adjuvants/liqvuid pesilicide formulations/alkylphenols (APs)/alkylphenol ethoxylates (APEOs)/high-performance liquid chromatography (HPLC)分类
农业科技引用本文复制引用
江泽军,曹晓林,李辉,张婵,金芬,佘永新,王静..液体农药制剂中烷基酚及其聚氧乙烯醚类助剂的高效液相色谱分析[J].农药学学报,2017,19(2):245-253,9.基金项目
科技部科技基础性工作专项项目(2013FY110100) （2013FY110100）
中国农业科学院科技创新工程"农业化学污染物残留检测及行为研究"创新团队. （）
