农药学学报2017，Vol.19Issue(2)：254-265,12.DOI:10.16801/j.issn.1008-7303.2017.0034
解淀粉芽孢杆菌TS-1203生防制剂中助剂和载体的筛选及配方优化
Screening and optimizing of the adjuvant and carriers for the bio-control agent Bacillus amyloliquefaciens TS-1203
摘要
Abstract
In order to develop highly effective and less toxic bio-control agent for the control of apple tree canker in vitro, the effect of surfactants, preservatives, UV-protectors and carriers on the growing strains of Bacillus amyloliquefaciens TS-1203, and the antibacterial activity of the B. amyloliquefaciens TS-1203 against Valsa mali were determined by the method of mycelium growth rate and response surface methodology(RSM). The results showed that Tween-80 was the most favorable surfactant for the bio-control agent at 0.50% mass fraction. Under this condition, the inhibition rate of TS-1203 was 88.90% at 48 h, which was not significantly different with that of the control. The sodium citrate wasthe most favorable preservative for the bio-control agent at 0.10% mass fraction. Under this condition, the highest inhibition rate of TS-1203 was 93.39% at 48 h, which was significantly higher than that of the control. Moreover, the sodium lignosulphonate was the most favorable ultraviolet protectant for the bio-control agent at 0.10% mass fraction. Under this condition, the inhibition rate of TS-1203 was 84.18% at 48 h, which was significantly higher than that of the control. The optimum adjuvant for the bio-control agent of TS-1203 was optimized by RSM, and the optimum adjuvant for 65 mL bacterial fermentation contained 650 μL of tween-80, 164.58 mg of sodium citrate and 308.12 mg of sodium lignosulfonate. The inhibitory rate of TS-1203 formulation against V. mali as high as 84.78%, under these conditions. In addition, the diatomite had the strongest effect on the growth of TS-1203, but it was easy to be layered. Sodium alginate had the strongest inhibitory effect on the growth of TS-1203. Therefore, the optimum carrier for bio-control agent TS-1203 was organic bentonite.关键词
解淀粉芽孢杆菌/苹果树腐烂病菌/TS-1203/助剂/载体/菌体活性Key words
Bacillus amyloliquefaciens/Valsa mali/TS-1203/additives/carrier/activity of the bacteria分类
化学化工引用本文复制引用
孙朝华,侯宝宏,张树武,薛应钰,张文军,徐秉良..解淀粉芽孢杆菌TS-1203生防制剂中助剂和载体的筛选及配方优化[J].农药学学报,2017,19(2):254-265,12.基金项目
公益性行业(农业)科研专项(201203034) （农业）
国家苹果产业技术体系(CARS-28) （CARS-28）
苹果主要病害生物防控技术研发与集成(2016YFD0201112)资助项目. （2016YFD0201112）
