青岛大学医学院学报2017，Vol.53Issue(1)：1-4,4.DOI:10.13361/j.qdyxy.201701001
3D打印纳米羟基磷灰石/聚己内酯支架生物相容性
BIOCOMPATIBILITY OF THREE-DIMENSIONAL PRINTED NANO-HYDROXYAPTITE/POLYCAPROLACTONE SCAFFOLD
摘要
Abstract
Objective To investigate the biocompatibility of three-dimensional (3D) printed nano-hydroxyaptite/polycaprolactone (nHA/PCL) scaffold.Methods Bone marrow mesenchymal stem cells (BMSCs) from Sprague-Dawely rats were isolated and co-cultured with 3D printed nHA/PCL scaffold,the growth and adhesion of cells on the scaffold surface were observed using scanning electron microscope (SEM).Applying CCK 8 assay,the cytotoxicity of 3D printed nHA/PCL scaffolds extract (experimental group),and DMEM medium (control group) on bone marrow mesenchymal stem cells was detected.Cell morphology of experimental group and control group was observed under an inverted phase contrast microscope.The extract of 3D printed nHA/PCL scaffolds on hemolytic reaction of fresh anticoagulant rabbit blood was detected-normal saline serving as negative control,and double distilled water as positive control-applying hemolysis test.Results The results of SEM showed that nHA/PCL scaffolds had a porous mesh structure,and BMSCs adhered and grew well on the surface of 3D printed nHA/PCL scaffolds.The results of inverted phase contrast microscope showed that cells grew well in 3D printed nHA/PCL scaffolds extract,and there was no difference in cell morphology between experimental group and control group.After 1,2,3 and 4 days of culture,the results of CCK8 showed the difference in absorbance value between the experimental group and the control group was not significant (P＞0.05),the cytotoxicity of scaffolds was grade 1.The results of hemolysis test showed the hemolysis ratio of 3D printed nHA/PCL scaffolds was 1.16％.Conclusion 3D printed nHA/PCL scaffold has good biocompatibility and it is expected to be a new material for repairing skull defect.关键词
组织支架/3D打印/聚己内酯/羟基磷灰石类/生物相容性试验Key words
tissue scaffolds/3D printing/polycaprolactone/hydroxyaptites/biocompatibility testing分类
医药卫生引用本文复制引用
余程,刘凯,侯长江,何涛,邓文帅,孙鹏..3D打印纳米羟基磷灰石/聚己内酯支架生物相容性[J].青岛大学医学院学报,2017,53(1):1-4,4.基金项目
国家自然科学基金资助项目(81671305) （81671305）
十二五国家科技支撑计划项目(2013BAI01B03) （2013BAI01B03）
山东省重点研发项目(2015-GSF118177) （2015-GSF118177）
青岛市自主创新重大科技专项(14-6-1-6-zdzx) （14-6-1-6-zdzx）
