青岛大学医学院学报2017，Vol.53Issue(1)：12-13,17,3.DOI:10.13361/j.qdyxy.201701004
枸橼酸铁铵对BV2小胶质细胞DMT1和FPN1表达影响
EFFECT OF FERRIC AMMONIUM CITRATE ON THE EXPRESSIONS OF DIVALENT METAL TRANSPORTER-1 AND FERROPORTIN-1 IN BV2 MICROGLIA
摘要
Abstract
Objective To investigate the impact of ferric ammonium citrate (FAC) on the expressions of divalent metal transporter 1 (DMT1) and ferroportin 1 (FPN1) in BV2 cells.Methods BV2 microglia cells were treated with 100μmol/L FAC for 24 h.Using Western Blot,the changes of DMT1 and FPN1 protein expression in BV2 microglia cells were observed.Results After 24 h treatment with FAC,the expression of DMT1 in BV2 microglia cells was lower than that in the control group (t =3.880,P＜0.01),and that of FPN1 was higher (t=4.409,P＜0.01).Conclusion After treatment with FAC for 24 h,the expression of DMT1 protein in BV2 microglia cells was down regulated,and that of FPN1 up regulated,this process may be related to the regulation of iron regulatory protein.关键词
枸橼酸铁铵/小神经胶质细胞/阳离子转运蛋白质类/铁转出蛋白1Key words
ferric ammonium citrate/microglia/cation transport proteins/ferroportin 1/分类
医药卫生引用本文复制引用
孟大鹏,谢俊霞,王俊..枸橼酸铁铵对BV2小胶质细胞DMT1和FPN1表达影响[J].青岛大学医学院学报,2017,53(1):12-13,17,3.基金项目
科技部国家重点基础研究发展计划973计划子课题项目(2011CB504102)与国家自然科学基金资助项目(31171031,81430024,31571054) （2011CB504102）
