青岛大学医学院学报2017，Vol.53Issue(1)：18-20,3.DOI:10.13361/j.qdyxy.201701006
Nesfatin-1对外侧臂旁核葡萄糖敏感性神经元兴奋性及大鼠摄食影响
EFFECTS OF NESFATIN-1 ON EXCITABILITY OF GLUCOSE-SENSITIVE NEURONS AND FEEDING IN LATERAL PARABRACHIAL NUCLEUS
摘要
Abstract
Objective To observe the effects of Nesfatin-1 on the excitability of glucose-sensitive neurons and feeding in the lateral parabrachial nucleus (LPBN),and explore the possible mechanism of Nesfatin-1 on feeding regulation.Methods Wistar rats were randomly divided into Nesfatin-1 group and normal saline (NS) group.Nocturnal food intake was measured by metabolic cage.Applying in vivo electrophysiological technique,the neurons were microinjection with glucose in LPBN to identify if they were glucose-sensitive neurons.Nesfatin-1 and NS were microinjected into glucose-sensitive neurons in LPBN to observe the impact of Nesfatin-1 and NS on neurons in LPBN.Results In Nesfatin-1 group,the six-hour and 12 hour cumulative food intake was lower than that in NS group (t =3.22,3.48;P＜0.05),and the difference of that in one-hour and four-hour food intake between the two groups was not significant (P＞0.05).There were glucose-sensitive neurons in LPBN,compared with NS group,Nesfatin-1 could significantly excite the glucose-inhibitory neurons in the LPBN (t=-4.99,P＜0.01).Conclusion Injection of Nesfatin-1 into lateral parabrachial nucleus can modulate the excitability of glucose-sensitive neurons in the nucleus and inhibit the nocturnal feeding in rats.关键词
臂旁核/Nesfatin-1/摄食行为/神经元/大鼠,WistarKey words
parabrachial nucleus/Nesfatin-1/feeding behavior/neurons/rats, Wistar分类
医药卫生引用本文复制引用
袁骏华,陈曦,韩文,王丽,蒋正尧,董静..Nesfatin-1对外侧臂旁核葡萄糖敏感性神经元兴奋性及大鼠摄食影响[J].青岛大学医学院学报,2017,53(1):18-20,3.基金项目
国家自然科学基金资助项目(31371168) （31371168）
