摘要
Abstract
Objective To investigate the neuroprotective effect of astaxanthin (AST) on chronic organophosphate poisoning (COP) mice Methods This study consisted of 30 adult healthy male Kunming mice.Adopting random number table method,eight mice were selected to serve as control group,and the other 22 mice were used to create a COP model with subcutaneous injection of Omethoate (5 mg/kg),once a day,for four weeks.After the models were completed,16 successful mouse models were selected and equally divided into model group and AST group in random.In AST group,AST was diluted with peanut oil to a concentration of 1 g/L,and 50 mg/kg,by gavage,once a day,was given to the mice for four weeks.Mice in the control and model groups were given equal volume of peanut oil by gavage for four weeks.Content of ROS in brain tissue of mice in each group was measured using enzyme-linked immunosorbent assay (ELISA),activity of glutathione peroxidase (GSH-PX) in hippocampus was assessed applying chemical colorimetry,morphology of hippocampus was observed by HE staining and apoptotic cells were counted employing terminal deoxynucleotidyl transferase dUTP nick end labeling assay (TUNEL).Results Compared with the control group,after COP,the content of ROS in brain tissue of mice in the model group was obviously higher (F=144.60,q=24.05,P＜0.05),the activity of GSH-PX was apparently lower (F =141.70,q =23.77,P＜0.05),the hippocampus neuronal injury was more obvious and the number of neuronal apoptosis was much increased (F =74.58,q =17.20,P ＜ 0.05).Compared with the model group,the ROS content in the AST group obviously declined (q =11.78,P＜0.05),the activity of GSH-PX significantly elevated (q=10.70,P＜0.05),neuronal damage and the number of apoptotic cells markedly reduced (q=9.94,P＜0.05).Conclusion AST exerts its antioxidant activity and inhibits apoptosis playing a neuroprotective role for chronic omethoate poisoning mice.关键词
虾青素/有机磷化合物/农药/活性氧/细胞凋亡Key words
astaxanthin/organophosphorus compounds/pesticides/reactive oxygen species/apoptosis分类
医药卫生
