日用化学工业（中英文）2017，Vol.47Issue(4)：219-222,4.DOI:10.13218/j.cnki.csdc.2017.04.008
睡莲花提取物对酪氨酸酶的抑制作用及其动力学研究
Inhibitory action of extracts from water lily flower on tyrosinase activity and its kinetics
摘要
Abstract
Tyrosinase inhibitory ratio of extracts from water lily (Nymphaea candida Presl. )flower (70%ethanol extract,NCE;30% ethanol elution parts,NCEA;50% ethanol elution parts,NCEB )were obtained based upon determination of dopa quinone with ultraviolet spectrophotometer. Furthermore,the effect of the extracts on monophenolase activity of tyrosinase and its inhibiting mechanism were examined by enzyme reaction kinetics method adopting L -tyrosine as substrate. Results show that these extracts can obviously inhibit activity of tyrosinase,of which NCEA displays the best inhibition effect as comparing with that of NCE and NCEB. The inhibition of NCEA on monophenolase activity of tyrosinase displays as reversible anti-competitive inhibition;while NCEB displays as reversible inhibition with mixed inhibition under low concentration condition,and competitive inhibition under high concentration condition.关键词
美白化妆品添加剂/睡莲花提取物/酪氨酸酶/抑制机制/动力学Key words
additive for whitening cosmetics/extracts of water lily (Nymphaea candida Presl. ) flower/tyrosinase/inhibition mechanism/kinetics分类
化学化工引用本文复制引用
李倩,李晨阳,詹羽娇,买吾拉江·阿不都热衣木,徐芳,赵军..睡莲花提取物对酪氨酸酶的抑制作用及其动力学研究[J].日用化学工业（中英文）,2017,47(4):219-222,4.基金项目
新疆维吾尔自治区公益性科研院所基本科研业务费专项资金资助项目(kygy2016166) （kygy2016166）
