四川医学2017，Vol.38Issue(4)：366-369,4.DOI:10.16252/j.cnki.issn1004-0501-2017.04.002
成都地区耐甲氧西林金黄色葡萄球菌的qacA基因检测及对常见消毒剂的抗性研究
Detection of qacA Gene in Methicillin-resistant Staphylococcus Aureus and Its Resistance to Common Disinfectants in Chengdu Area
摘要
Abstract
Objective To investigate the status of the disinfectant resistant gene qacA in clinical isolates of methicillin-resistant Staphylococcus aureus(MRSA)and the resistance to common disinfectants.Methods PCR method was used to detect the carrying capacity of qacA gene of 86 strains of MRSA.Broth dilution method was used to detect the MBCof common disinfectants against MRSA strain.Results 1 qacA positive strainwas detected in all the 86 strains of MRSA,and the positive rate was 1.2%.The MBC50 of benzalkonium bromide,three cyanuric acids,and glutaraldehyde for all MRSA strains were respectively 64μg/mL,256μg/mL,and 256μg/mL.Conclusion MRSA strain carrying disinfectant resistant gene qacA is not prevalent in this area.The local MRSA strains of chlorine containing disinfectant,disinfectant aldehydes show high resistance level.关键词
耐甲氧西林金黄色葡萄球菌/qacA基因/消毒剂抗性Key words
methicillin resistant staphylococcus aureus/qaca gene/disinfectant resistance分类
医药卫生引用本文复制引用
纪风兵,熊杰,胡章勇,邹自英,朱义芳,王丹,刘媛,刘晨霞..成都地区耐甲氧西林金黄色葡萄球菌的qacA基因检测及对常见消毒剂的抗性研究[J].四川医学,2017,38(4):366-369,4.基金项目
四川省卫生厅资助科研课题(编号:130382) （编号:130382）
