山东农业大学学报（自然科学版）2017，Vol.48Issue(2)：161-165,5.DOI:10.3969/i.issn.1000-2324.2017.02.001
紫花地丁提取物对三文鱼中副溶血性弧菌的抑制作用
The Bacteriostasis of Extracts from Viola yedoensis against Vibrio parahaemolyticus in Salmon
摘要
Abstract
In order to obtain the natural active substances with antibacterial activity that can be used in food,the bacteriostasis against Vibrio parahaemolyticus and extracts from salmon were studied.The extracts of Viola yedoensis were examined by L16(43) orthogonal design with the use of combinations of ethanol concentration,ultrasound time and ultrasound temperature levels.The optimal extraction conditions were 75％ (w/w) ethanol and ultrasound 20 min at 40 ℃.With this extraction condition,the MBC (Minimum Bactericidal Concentration) and MIC (Minimum Inhibitory Concentration) of the extract for V.parahaemolyticus inoculated with salmon were 0.50 mg/g and 6.25* 10-2 mg/g,respectively.The extracts could be provided a prospect for a application in in food.关键词
紫花地丁/提取条件/副溶血性弧菌/三文鱼/抑菌Key words
Viola yedoensis/extraction condition/Vibrio parahaemolyticus/salmon/bacteriostasis分类
轻工纺织引用本文复制引用
施杨,郭法利,刘海泉..紫花地丁提取物对三文鱼中副溶血性弧菌的抑制作用[J].山东农业大学学报（自然科学版）,2017,48(2):161-165,5.基金项目
国家自然科学基金面上项目(31671779) （31671779）
上海市自然科学基金项目(15ZR1420600) （15ZR1420600）
上海海洋大学博士科研启动资金(A2-0203-17-100316) （A2-0203-17-100316）
