山东农业大学学报（自然科学版）2017，Vol.48Issue(2)：203-209,7.DOI:10.3969/j.issn.1000-2324.2017.02.008
响应面法优化酶辅助提取废弃烟叶中烟碱
Optimization of Nicotine Extracted from Abandoned Tobacco with Response Surface Method
摘要
Abstract
To optimize the conditions for purification of Nicotine in abandoned Tobacco.Response surface method was used to design the test models with four factors including enzyme time,enzyme temperature,enzyme dosage,ultrasonic time at three levels based on the single factor experiment and the yield of Nicotine was used as the evaluation index to optimize purification process of Nicotine.The optimum extraction condition were as follows:enzymolysis time was 2.00 h,enzymolysis temperature was 48.50 ℃,cellulose enzyme amount was 2.10％,pH was 4.00.Under this condition,the yield was up to 3.320％,the relative error was smaller compared to the predicted value 3.32836％.The optimum extraction conditions were simple and stable and they were served as a reference for industrial large scale production.关键词
烟碱/废弃烟叶/响应面法Key words
Nicotine/abandoned tobacco/response surface method分类
农业科技引用本文复制引用
吴映捷,吕新美,闫兵兵,胡鲲,杨先乐..响应面法优化酶辅助提取废弃烟叶中烟碱[J].山东农业大学学报（自然科学版）,2017,48(2):203-209,7.基金项目
国家高技术研究发展计划(863计划)项目(2011AA10A216) （863计划）
公益性行业(农业)科研专项经费(201203085) （农业）
