山东农业科学2017，Vol.49Issue(1)：7-12,6.DOI:10.14083/j.issn.1001-4942.2017.1.002
小菜蛾PxALP1基因的克隆与生物信息学分析
Cloning and Bioinformatic Analysis of PxALP1 Gene from Plutella xylostella
摘要
Abstract
The diamondback moth,Plutdla xylostella (L.),is one of the world wide agricultural pests of cruciferous vegetables,and is also one of the earliest populations which produce resistance to pollution-free biological pesticide Bt toxin.The membrane bound alkaline phosphatase has been shown to be a direct receptor of Bt toxin protein in many insects according to previous studies.However,the coding genes and biological functions of Plutella xylostella alkaline phosphatase is rarely known.In this study,the open reading frame (ORF) of PxALP1 gene was cloned and the protein primary structure was deduced.A classic alkPPc (alkaline phosphatase homologues) domain was found in the 79th ～ 513th amino acid residue by SMART analysis,and a membrane bound domain was found through SWISS-MODEL homology model construction.Both of them indicated that PxALP1 was a potential membrane bound alkaline phosphatase.Phylogenetic analysis showed that diamondback moth PxALP1 and membrane combine alkaline phosphatase of Lepidoptera insect silkworm had the closest relationship.BLAST aligning exhibited that the identity between PxALP1 and silkworm BmAlpm was 36.3 ％ and the similarity was 50.3 ％,which proved that there was high conservation between them.关键词
小菜蛾/ALP1/碱性磷酸酶/生物信息学分析Key words
Plutella xylostella/ALP1/Alkaline phosphatase/Bioinformatic analysis分类
农业科技引用本文复制引用
胡霞,刘晓琳,白义川,徐维红,刘佰明,邹德玉,许静杨,谷希树..小菜蛾PxALP1基因的克隆与生物信息学分析[J].山东农业科学,2017,49(1):7-12,6.基金项目
天津市植物保护研究所所长基金项目(201402) （201402）
