山东农业科学2017，Vol.49Issue(1)：136-140,5.DOI:10.14083/j.issn.1001-4942.2017.1.029
山东东南沿海绿茶成分的UHPLC-MS/MS分析
UHPLC-MS/MS Analysis of Main Components of Green Tea in Southeast Coast of Shandong
摘要
Abstract
The qualitative and quantitative analysis of 5 kinds of green tea in the southeast coast of Shandong were conducted by ultrahigh-performance LC and tandem mass spectrometry (UHPLC-MS/MS) in this study.The smashed tea samples were analyzed after ultrasonic extraction in 50％ methanol (V/V).And the separations were carried out using Hypersil GOLD C18 chromatographic column through gradient elution with 0.05 ％ formic acid aqueous and acetonitrile as moving phase.The contents of 7 catechins in 5 kinds of Shandong green tea were quantified,and 24 compounds were identified.The composition of catechins in fresh leaves of Shandong green tea was similar,while the contents differed.The contents of EGCG,EGC,GC,EC,C and ECG in Shandong Zhulu were higher than those of the other 4 kinds of green tea,while the content of GCG was the highest in Shandong Jufeng.Meanwhile,among the 24 compounds,two kinds of procyanidins dimmers were less reported.关键词
山东绿茶/主要成分/UHPLC-MS/MSKey words
Shandong green tea/Main components/UHPLC-MS/MS分类
农业科技引用本文复制引用
房泽海,孟启路..山东东南沿海绿茶成分的UHPLC-MS/MS分析[J].山东农业科学,2017,49(1):136-140,5.基金项目
山东省高等学校自然科学研究项目“山东道地绿茶的品质特性及降血脂功能研究”(J13LM56) （J13LM56）
淄博市科学技术发展计划“固定化黑曲霉发酵绿茶废料高产ECGG的研究”(2013GG01235) （2013GG01235）
山东省科技发展计划项目“山东绿茶的品质特征研究及质量标准体系的建立”(2014GNC110032) （2014GNC110032）
