山东医药2017，Vol.57Issue(7)：1-4,4.DOI:10.3969/j.issn.1002-266X.2017.07.001
原发性肝癌患者癌组织、血清miR-202表达变化及其意义
Expression changes of miR-202 in cancerous tissues and serum of patients with primary hepatocellular carcinoma
摘要
Abstract
Objective To observe the expression changes and clinical significance of microRNA-202 (miR-202) in the cancerous tissues and serum of patients with primary hepatocellular carcinoma (HCC).Methods We collected 70 cases of primary HCC tissues and preoperative serum from 70 HCC patients,30 cases of normal liver tissues which were confirmed with no tumor infiltration by pathology from 30 hepatic hemangioma patients,as well as 30 cases of serum from 30 healthy volunteers.RT-PCR was used to detect the expression of miR-202 in the normal liver tissues and primary HCC tissues,and in the serum of HCC patients and healthy volunteers,respectively.The relationship between miR-202 expression and the clinicopathological parameters in HCC patients was analyzed.We drew the ROC curve to evaluate the diagnostic value of miR-202,AFP,miR-202 combined with AFP in primary liver cancer.Results The relative expression of miR-202 in the primary HCC tissues was lower than that in the normal liver tissues (all P ＜ 0.05).The relative expression of miR202 in the serum of HCC patients was lower than that in healthy subjects (P ＜ 0.05).The relative expression of miR-202 in primary HCC tissues was higher than that in the serum of HCC patients (P ＜ 0.05).The expression of miR-202 in primary HCC tissues was not correlated with gender,age,serum AFP level,tumor diameter,tumor size,tumor location,TNM stage,metastasis,invasion,HBV infection,with or without liver cirrhosis and portal vein thrombosis in HCC patients (all P ＞ 0.05).The serum levels of miR-202 in HCC pateints were only correlated with the number of tumors (P ＜ 0.05),but were not correlated with sex,age,serum AFP,tumor diameter,tumor number,tumor location,TNM stage,metastasis or not,capsule infiltration,HBV infection,with or without liver cirrhosis and portal vein thrombosis (all P ＞ 0.05).Combined detection of miR-202 and tumor marker AFP could get the higher sensitivity (81.8％) and specificity (93.0％) in the diagnosis of HCC,the AUC was 0.942.Conclusions The miR-202 expression in HCC tissues and serum is significantly lower in HCC patients,the miR-202 expression in HCC serum is correlated with tumor number,and the combined detection of miR-202 and AFP could get the higher sensitivity and specificity in the diagnosis of HCC.关键词
肝癌/原发性肝癌/肝肿瘤/微小RNA/微小RNA202Key words
hepatocellular carcinoma/primary hepatocellular carcinoma/liver neoplasm/microRNA/microRNA 202分类
医药卫生引用本文复制引用
於琳,张开炯,柴利,李宝林,杨志惠,刘靳波..原发性肝癌患者癌组织、血清miR-202表达变化及其意义[J].山东医药,2017,57(7):1-4,4.基金项目
四川省科学技术厅基金资助项目(14JC0801) （14JC0801）
西南医科大学附属医院科研项目(2015-QS-017). （2015-QS-017）
