山东医药2017，Vol.57Issue(7)：5-8,4.DOI:10.3969/j.issn.1002-266X.2017.07.002
芒柄花黄素对人胃癌细胞株MKN-45增殖、凋亡的影响及其机制
Effects of formononetin on proliferation and apoptosis of human gastric cancer MKN-45 cells
摘要
Abstract
Objective To observe the effects of formononetin on the proliferation and apoptosis of human gastric cancer MKN-45 cells and its possible mechanism.Methods The human gastric cancer MKN-45 cells in the logarithmic growth phase were divided into groups A,B,C and the control group.Cells in the groups A,B and C were cultured and the culture medium was added with 20,40 and 80 μg/mL of formononetin,respectively.The control group was not treated.The cell proliferation of MKN-45 cells was detected by MTT assay at 24,48 and 72 h of formononetin treatment,and the growth inhibitory rate of MKN-45 cells was assessed.The effect of formononetin on morphological changes was evaluated using DAPI staining.The intracellular phosphorylated IκB (p-IκB) and NF-κB p65 protein expression was measured by Western blotting.Results MKN-45 cell proliferation was inhibited after being treated by formononetin with a time-and dose-dependent manner (all P ＜ 0.05).The apoptosis rates of groups A,B,C and the control group after 72-hour treatment were 25.62％ ± 2.57％,48.27％ ± 3.18 ％,72.51％ ± 4.35％ and 1.07％ ± 0.54％,respectively (all P ＜ 0.05).The expression of p-IκB and NF-κB p65 protein in cells of groups A,B and C was significantly higher than that of the control group at 48 h after administration with a dose-dependent manner (all P ＜ 0.05).Conclusion Formononetin inhibits cell proliferation and induces apoptosis of MKN-45 cells,and the possible mechanism may be related to activation of NF-κB signaling pathway.关键词
芒柄花黄素/胃癌/胃肿瘤/细胞增殖/细胞凋亡Key words
formononetin/gastric carcinoma/stomach neoplasms/cell proliferation/apoptosis分类
医药卫生引用本文复制引用
董陈诚,钟漓,张广钰,王振冉,冉福林,陈霄..芒柄花黄素对人胃癌细胞株MKN-45增殖、凋亡的影响及其机制[J].山东医药,2017,57(7):5-8,4.基金项目
广西高校科学技术研究项目(LX2014270). （LX2014270）
