山东医药2017，Vol.57Issue(7)：13-15,3.DOI:10.3969/j.issn.1002-266X.2017.07.004
宽筋藤提取物对高尿酸血症小鼠血清尿酸水平的影响及对大鼠痛风性炎症的预防作用
Effect of extract from tinospora cordifolia on serum uric acid level of hyperuricemia in mice and its preventive effect on gouty inflammation in rats
摘要
Abstract
Objective To observe the effect of the extract from tinospora cordifolia on serum uric acid level of hyperuricemia in mice and its preventive effect on gouty inflammation in rats.Methods One hundred male mice were divided into two groups:groups A and B,50 in each.Fifty mice in the group A were used to make the hyperuricemia models,and then they were divided into 5 secondary groups.Mice in 3 of the 5 groups were given 10.0,5.0 and 2.5 g/kg tinospora cordifolia extract by garage.Mice in the other two secondary groups were given allopurinol tablets and equal volume of distilled water by gavage,respectively.The rest of 50 mice in the group B were taken as the control group and then were divided into 5 secondary groups.Mice in 3 of the 5 groups were given high-does,medium-dose and low-dose tinospora cordifolia extract by gavage.The other two groups were given 20 mg/kg allopurinol tablets and the same volume of distilled water by garage respectively for ten days (1 time per day).The serum uric acid level was tested on the 10th day.Anther 40 male rats were randomly divided into 4 groups:groups 1,2,3 and 4.Rats in the groups 1 and 2 were given high-dose and low-dose allopurinol tablets by gavage for 12 days (1 time per day).Rats in the groups 3 and 4 were given equal volume of distilled water for 12 days (1 time per day).Rats in the groups 1,2,3 were given 0.15 mL 100 g/L MSU by subcutaneous injection on right hind plant for the gout models.At 1,2,3,4,5,6 h before and after the injection,the swelling of the right hind paw was observed and the degree of swelling was calculated.Results The serum uric acid level of the three secondary groups of group A which were given high-dose and medium-dose tinospora cordifolia extract were lower than that of the secondary group given distilled water all P ＜ 0.05).The swelling degree of the group 3 was higher than that of the group 4 (all P ＜ 0.05).After 1 and 3 hours of the subcutaneous injection,the swelling degree of groups 1 and 2 was lower than that of group 3 (all P ＜ 0.05).After 2 and 5 hours of the subcutaneous injection,the swelling degree of groups 1 and 2 was higher than that of the group 3 (all P ＜ 0.05).After 4 hours of the subcutaneous injection,the swelling degree of group 1 was lower than that of group 3 (all P ＜ 0.05),while the swelling degree of the group 2 was higher than that of the group 3 (all P ＜ 0.05).After 6 hours of the subcutaneous injection,the swelling degree of group 1 was lower than that of group 3 (all P ＜ 0.05).Conclusion The extract of tinospora cordifolia can decrease the serum uric acid level of hyperuricemia in mice and prevent the gouty inflammation in rats.关键词
宽筋藤提取物/痛风/尿酸/高尿酸血症/痛风性炎症Key words
tinospora cordifolia extracts/gout/uric acid/hyperuricemia/gouty inflammation分类
医药卫生引用本文复制引用
张柯媛,刘喜华,覃艺群,熊灏,刘安韬,周小雅..宽筋藤提取物对高尿酸血症小鼠血清尿酸水平的影响及对大鼠痛风性炎症的预防作用[J].山东医药,2017,57(7):13-15,3.基金项目
广西自然科学基金资助项目(2014GXNSFBA118202 （）
2015GXNSFBA139150). （）
