山东医药2017，Vol.57Issue(9)：5-8,4.DOI:10.3969/j.issn.1002-266X.2017.09.002
Psammaplin A对离体结肠癌SW480细胞增殖、凋亡的影响及机制
Effect of Psammaplin A on proliferation and apoptosis of colon cancer SW480 cells in vitro
摘要
Abstract
Objective To observe the effect of histone deacetylase 3 (HDAC3) inhibitor Psammaplin A on the proliferation and apoptosis of colon cancer cells in vitro.Methods Colon cancer SW480 cells in logarithmic phase were randomly divided into Psammaplin group and control group.Different concentrations of Psammaplin A (0.5,5,50,500 and 5 000 μg/mL) were given and interfered for 48 h to cells in the Psammaplin A group,and no interference was given to cells in the control group.HDAC3 and DNMT3a protein expression was detected by Western blotting.MTT assay was used to detect the proliferation of SW480 affected by Psammaplin A in different concentrations and culture time (24,48,72,96 h).Flow cytometer was used to detect the cell cycle and apoptosis affected by Psammaplin A.Results HDAC3 relative protein expression in the Psammaplin group which was interfered by 0.5,5,50,500 and 5 000 μg/mL Psammaplin A for 48 h was 23.28 ± 8.91,21.72 ± 9.18,18.63 ± 7.26,14.17 ± 5.64,10.58 ± 7.22,and 24.84 ± 7.65 in the control group.Compared with control group,the differences were significant in the Psammaplin at 500 and 5 000 μg/mL (all P ＜0.05).DNMT3a relative protein expression in the Psammaplin group which was interfered by 0.5,5,50,500 and 5 000 μg/mL Psammaplin A for 48 h was 8.36 ± 8.43,17.51 ± 6.29,16.12 ± 6.54,1 1.27 ± 5.31 and 10.54 ± 4.26,and 20.15 ± 6.31 in the control group.Compared with contml group,the differences were significant in the Psammaplin at 500 and 5 000 μg/mL (all P ＜ 0.05).After SW480 cells were interfered with 50 μg/mL Psammaplin A and cultured for 24,48,72 and 96 h,the survival rate in the Psammaplin group was decreased in a time-dependent manner.Compared with the control group,the difference was significant at 48,72 and 96 h (all P ＜ 0.05).The survival rate in the Psammaplin group treated with 0.5,5,50,500 and 5 000 μg/mL Psammaplin A for 48 h was decreased in a dose-dependent manner,and the different was significant at 50,500 and 5 000 μg/mL as compared with survival rate in the control group (all P ＜0.05).The percentages of cells in G1 phase of the Psammaplin group and control group were 69.27％ ± 0.93％ and 81.25％ ±0.89％,respectively,and those in the G2 phase were 4.72％ ± 1.83％ and 9.62％ ± 1.34％,in S phase were 27.61％ ± 1.65％ and 10.43％ ± 0.97％,and the differences were significant (all P ＜ 0.05).The apoptosis rate in the Psammaplin group and control group was 56.98％ and 31.67％,respectively,and the difference was significant (P ＜0.01).Conclusion Psammaplin A decreases the survival rate and proliferation of colon cancer cells by inhibiting the expression of HDAC3 and DNMT3a,and induces apoptosis.关键词
结肠癌/Psammaplin A/组蛋白去乙酰化3蛋白/DNA甲基化3a蛋白Key words
colon carcinoma/Psammaplin A/histone deacetylase 3 protein/DNA methyltransferase 3a protein分类
医药卫生引用本文复制引用
刘海宏,孟庆凯,石刚..Psammaplin A对离体结肠癌SW480细胞增殖、凋亡的影响及机制[J].山东医药,2017,57(9):5-8,4.基金项目
辽宁省自然科学基金资助项目(2014020102) （2014020102）
辽宁省省直医院临床能力建设青年项目(LNCCC-D46-2015). （LNCCC-D46-2015）
