山东医药2017，Vol.57Issue(9)：12-15,4.DOI:10.3969/j.issn.1002-266X.2017.09.004
小鼠结直肠癌CT26细胞WTX基因稳定干扰系的建立
Establishment of a mouse colorectal cancer CT26 cell line with stable interference of WTX gene
摘要
Abstract
Objective Using a WTX gene recombinant lentivirus interference vector to infect the mouse colorectal cancer CT26 cell hne to establish a mouse colorectal cancer CT26 cell line with stable interference of WTX gene.Methods The recombinant lentiviral was transformed in mouse colorectal cancer CT26 cells,and fluorescence microscopy was used to observe the preliminary infection efficiency.The stably transfected ceils were selected with G418.The cellular expression of WTX mRNA was detected using quantitative PCR and the cellular expression of WTX protein was detected by immunohistochemistry and Western blotting.Results After the mouse colorectal cancer CT26 cells were infected by lentiviral,the expression levels ofWTX mRNA and protein were significantly decreased in CT26 cells,indicating a mouse colorectal cancer CT26 cell line with stable interference of WTX gene was successfully established.Conclusion We successfully establisheda mouse colorectal cancer CT26 cell line with stable interference of WTX by lentiviral infection.关键词
结直肠癌/WTX基因/慢病毒载体/稳定转染Key words
colorectal carcinoma/WTX gene/lentivirus vector/stable transfection分类
医药卫生引用本文复制引用
王蕾,张庆玲..小鼠结直肠癌CT26细胞WTX基因稳定干扰系的建立[J].山东医药,2017,57(9):12-15,4.基金项目
国家自然科学基金资助项目(81272760 （）
81472712). （）
