山东医药2017，Vol.57Issue(9)：16-19,4.DOI:10.3969/j.issn.1002-266X.2017.09.005
微小RNA-200c靶向抑制EFNA1基因对胃癌细胞增殖、凋亡及侵袭的影响
Effect of Micro-200c on cell proliferation, apoptosis and invasion of gastric cancer by targeting EFNA1 gene
摘要
Abstract
Objective To evaluate the effect of microRNA (miR)-200c on regulating cell proliferation,apoptosis and invasion by targeting ephrin A1 (EFNA1) in gastric cancer cells.Methods SGC7910 cells were cultivated and randomly divided into miR-200c mimics group and mimics control group,which were then transfected by miR-200c mimics and mimics oligonucleotides.Cell proliferation was detected by CCK-8,apoptosis was determined by flow cytomet~,and cell invaswn was assessed using Transwell assay.The target relationship between miR-200c and EFNA1 was measured by the dual luciferase reporter gene system.Cancer and adjacent non-cancerous tissues were obtained from 48 patients who underwent gastrectomy at Guangzhou First People's Hospital,and the protein expression of EFNA1 was tested by immunohistochemistry.The relationship between EFNA1 expression and gender,age,smoking status,alcohol drinking,histological type,depth of invasion,lymph node and liver metastasis was assessed.Results Compared with the mimics control group,the proliferative activity of SGC7910 cells in the miR-200c mimics group was decreased.the whole apoptosis rate was increased and the invasive ability was decreased (all P ＜ 0.05).The luminescence intensity was significantly decreased in miR-200c mimic transfected cells than in mimics control cells (P ＜ 0.05),and the luminescence intensity was significantly increased in miR-200c inhibitor transfected cells than in inlhibitor control cells (P ＜ 0.05).EFNA1 was significantly up-regulated in the gastric cancer tissues as compared with that noted in the adjacent non-cancerous tissues.The high expression of EFNA1 was associated with lymph node metastasis (P ＜ 0.05),bat not associated with other clinicopathological characteristics (all P ＞ 0.05).Conclusion EFNA1 gene was highly expressed in the gastric cancer.miR-200c can inhibit gastric cancer cell proliferation and invasion,and enhance cell apoptosis by targeting EFNA1 gene.关键词
胃癌/微小RNA-200c/肝配蛋白A1/细胞增殖/侵袭Key words
gastric carcinoma/micro RNA-200c/ephrin A1/cell proliferation/invasion分类
医药卫生引用本文复制引用
李鹰飞,高健伟,王红,周永健,聂玉强..微小RNA-200c靶向抑制EFNA1基因对胃癌细胞增殖、凋亡及侵袭的影响[J].山东医药,2017,57(9):16-19,4.基金项目
国家自然科学基金资助项目(81502040) （81502040）
广州市卫生和计划生育科技一般引导项目(20161A010009). （20161A010009）
