山东医药2017，Vol.57Issue(9)：20-23,4.DOI:10.3969/j.issn.1002-266X.2017.09.006
活动性系统性红斑狼疮患者外周血嗜碱性粒细胞CD62L、BAFF和CD40L表达变化及意义
Expression changes of CD62L, BAFF and CD40L in basophils of patients with systemic lupus erythematosus
摘要
Abstract
Objective To investigate expression changes of lymph homing receptor (CD62L and B-lymphocyte activation factors (BAFF and CD40L) in peripheral blood basophils of patients with active systemic lupus erythematosus (SLE) and their significance.Methods Fifty-seven SLE patients were selected and were divided into the active group (SLEDAI score≥10,n =29) and inactive group (SLEDAI score ＜ 10,n =28) according to the SLEDAI score.Twenty healthy volunteers were selected as the control group.The levels of CD62L,BAFF and CD40L in the peripheral blood basophils were detected by flow cytometry and their correlations with the Serum anti-dsDNA levels were analyzed by Spearman correlation analysis.Results The expression of CD62L in peripheral blood basophils of the active group and inactive group was higher than that of the control group,and the active group was significantly higher than the inactive group (P ＜0.05).The expression of BAFF and CD40L in peripheral blood basophils of the active group was significantly higher than that of the inactive group and control group (all P ＜ 0.05),while there was no significant difference between inactive group and control group (P ＞ 0.05).Expression of CD62L and CD40L in the peripheral blood basophils was positively correlated with serum anti-dsDNA levels in SLE patients (r =0.34,r =0.36;all P ＜ 0.05).The expression of BAFF in peripheral blood basophils was not related with serum anti-dsDNA levels in SLE patients (r =0.26,P =0.14).Conclusions Expression of CD62L,BAFF and CD40L in basophils of patients with active SLE increases.CD62L may mediate the basophils migrating to the secondary lymph organs where the basophils could activate the self-reactive B-lymphocytes producing diverse antibodies through BAFF and CD40L to participate in the pathogenesis of SLE.关键词
系统性红斑狼疮/嗜碱性粒细胞/嗜碱性粒细胞淋巴归巢受体/B淋巴细胞活化刺激因子Key words
systemic lupus erythematosus/basophil/CD62L/B-lymphocyte activation factors分类
医药卫生引用本文复制引用
冯永民,陈秋萍,黄柳涛,潘庆军,刘华锋..活动性系统性红斑狼疮患者外周血嗜碱性粒细胞CD62L、BAFF和CD40L表达变化及意义[J].山东医药,2017,57(9):20-23,4.基金项目
国家自然科学基金资助项目(81202346) （81202346）
广东省自然科学基金项目(S2012040006216) （S2012040006216）
广东省医学科学技术研究基金项目(A2015520). （A2015520）
