山东医药2017，Vol.57Issue(9)：24-27,4.DOI:10.3969/j.issn.1002-266X.2017.09.007
CPEB1对人脐静脉血管内皮细胞增殖和迁移的影响及机制
Effect of CPEB1 on proliferation and migration of HUVECs
摘要
Abstract
Objective To investigate the effect of cytoplasmic polyadenylation-element-binding protein 1 (CPEB1) on the proliferation and migration of human umbilical-vein endothelial cells (HUVECs) and its possible mechanism.Methods HUVECs in logarithmic phase were divided into experimental group and control group,which were cultured with tumor cell supernatant medium and the common medium,respectively.Real-time PCR and Western blotting were used to detect the mRNA and protein expression of CPEB1 and a disintegrin and metalloproteinase 17 (ADAM17).HUVECs in logarithmic phase with 70％-80％ cells conjugation were transfected by LipofectamineTM2000.Cells were then divided into three group:carrying gene plasmid transfection group (transfection group),without empty plasmid gene transfection group (control group) and without any processing cell group (blauk control group).The proliferation ability of HUVECs after transfection was determined by MTT,the migration ability of HUVECs was detected by Transwell chamber,and RT-PCR was used to detect ADAM17 mRNA expression and Western blotting to detect ADAM17 protein expression.Results CPEB1 mRNA and protein expression levels in the experimental group were lower than those of the control group (all P ＜ 0.05).ADAM17 mRNA and protein the expression levels in experimental group were higher than those of the control group (all P ＜0.05).At 48 h and 72 b after transfection,the absorbance value (A) of the transfection group was obviously lower than those of control group blank control group (all P ＜ 0.05).The number of cells through the small chamber membrane in the transfection group was lower than that in the control group and blank control group (all P ＜ 0.05).ADAM17 mRNA and protein levels in the transfection group was lower than that in the control group and blank control group (all P ＜ 0.05).Contusion The supematant of tumor cells can induce the change in expression of CPEB1 and ADAM17 in HUVECs,and over-expression of CPEB1 may inhibit the proliferation and migration of HUVECs by regulating ADAM17.关键词
人脐静脉血管内皮细胞/胞质多聚腺苷酸结合蛋白1/整合素-金属蛋白酶17/细胞增殖/细胞迁移Key words
human umbilical-vein endothelial cells/cytoplasmic polyadenylation-element-binding protein 1/a disintegrin and metalloproteinase 17/cell proliferation/cell migration分类
医药卫生引用本文复制引用
范良,殷铁军..CPEB1对人脐静脉血管内皮细胞增殖和迁移的影响及机制[J].山东医药,2017,57(9):24-27,4.基金项目
华中科技大学同济医学院附属武汉中心医院院内科研基金项目(YQ14A03). （YQ14A03）
