山东医药2017，Vol.57Issue(10)：1-4,4.DOI:10.3969/j.issn.1002-266X.2017.10.001
白芍总苷对肝癌耐药细胞BEL-7402/ADM耐药性的逆转作用及机制探讨
Reversing effects of total glucosides of paeony on drug resistance of BEL-7402/ADM cells
摘要
Abstract
Objective To observe the reversing effects of total glucosides of paeonia (TPG) on drug resistance of BEL-7402/ADM cells in hepatocellular carcinoma.Methods Human hepatocellular carcinoma ADM sensitive BEL-7402 cells (parent group) and ADM drug-resistant BEL-7402/ADM cells (drug resistance group) were cultured in vitro.BEL-7402/ADM cells were transfected with HDAC3 siRNA interference plasmid (interference group) and empty plasmid (empty plasmid group) for 24 h,respectively,and BEL-7402/ADM cells were incubated with TPG for 48 h (drug group).Different concentrations of ADM were administered to cells in the parent group,drug resistance group and drug group for 48 h respectively.Then MTT assay was used to detect the growth inhibition rates and IC50 of ADM in each group.The mRNA and protein expression of MDR1 and histone deacetylase 3 (HDAC3) was detected by RT-qPCR and Western blotting in the above grovps.Results IC50 of ADM was 0.139 nmoL/L in the parent group,8.807 nmoL/L in the drug resistance group and 4.890 nmoL/L in the drug group.Drug reverse ratio was about 1.80 times higher and the reverse transcription ratio was 45.23％ in the drug group.The relative expression of MDR1 mRNA and protein was:drug resistance group,empty plasmid group ＞ drug group ＞ interference group ＞ parent group (all P＜0.05,0.01),the relative expression of HDAC3 and protein was:drug resistance group,empty plasmid group ＞ interference group ＞ parent group,drug group (all P＜0.05,0.01).Conclusion TPG induces the down-regulated expression of MDR1 by inhibiting the HDAC3 expression,thus reversing the drug resistance of BEL-7402/ADM cells.关键词
肝癌/白芍总苷/耐药/siRNA干扰/半数抑制浓度/多药耐药基因/去乙酰化酶Key words
hepatoma carcinoma/total glucosides of paeony/drug resistance/siRNA interference/50％ inhibition concentration/multidrug resistance gene/histone deacetylase分类
医药卫生引用本文复制引用
胡礼仪,侯永彬,余莉华,米永华,王科,刘北忠..白芍总苷对肝癌耐药细胞BEL-7402/ADM耐药性的逆转作用及机制探讨[J].山东医药,2017,57(10):1-4,4.基金项目
重庆医科大学附属永川医院引进人才资助项目(YJYJ201306). （YJYJ201306）
