山东医药2017，Vol.57Issue(10)：8-10,3.DOI:10.3969/j.issn.1002-266X.2017.10.003
Wnt信号通路阻滞剂DKK-1对人结肠癌细胞HCT116侵袭能力的影响
Effects of Wnt signaling pathway antagonist DKK-1 on invasion ability of colon cancer cell line HCT116
摘要
Abstract
Objective To investigate the effects and molecular mechanism of Wnt signal pathway antagonist DKK-1 on invasion ability of colon cancer cell line HCT116.Methods Conlon cancer HCT116 cells were divided into the experimental group and control group,which were cultured with exogenous DKK-1 (100 ng/mL) and the same amount of cell culture medium for 48 h,respectively.Transwell was used to observe the change of invasion ability.The protein and mRNA expression of β-catenin,Snail,E-cadherin and Vinentin in the HCT116 cells was observed by Western blotting and RT-PCR.Results The number of transmembrane cells in the experimental group was (45.29 ± 6.25)/HP which was obviously lower than that in control group [(76.36±8.32)/HP],and the difference was statistically significant (P ＜ 0.05).The protein and mRNA expression of β-catenin,Snail and Vinentin was lower and the expression of E-cadherin protein and mRNA was higher in the experimental group than that of the control group,and the differences were statistically significant (all P＜0.05).Conclusion Wnt signal pathway antagonist DKK-1 can decrease the invasion ability of conlon cancer cell line HCT116 by inhibiting the process of epithelial-mesenchymal transition in tumor cells.关键词
结肠癌/Wnt信号通路/通路阻滞剂/上皮-间充质转化/细胞侵袭Key words
colon carcinoma/Wnt signaling pathway/pathway antagonist/epithelial-mesenchymal transition/cell invasion分类
医药卫生引用本文复制引用
张延新,张印坡,赵琰,王记红,张艳,崔东涛..Wnt信号通路阻滞剂DKK-1对人结肠癌细胞HCT116侵袭能力的影响[J].山东医药,2017,57(10):8-10,3.基金项目
河南省教育厅青年教师资助项目(2012GGJS-269). （2012GGJS-269）
