山东医药2017，Vol.57Issue(16)：16-19,4.DOI:10.3969/j.issn.1002-266X.2017.16.005
氯化锂对激素性股骨头坏死大鼠股骨头微观结构的影响
Effects of lithium chloride on microstructure of femoral head in rats with steroid-induced osteonecrosis of the femoral head
摘要
Abstract
Objective To investigate the effect of lithium chloride on microstructure of femoral head in rats with steroid-induced osteonecrosis of the femoral head (S-ONFH) and its significance.Methods Thirty SD rats were randomly divided into the blank control group, model group and lithium chloride group, with 10 rats in each group.In the lithium chloride group and model group, we used escherichia coli endotoxin combined with large dose of steroid shock to make the rat models of the S-ONFH.At 24 h after the last injection of steroid, rats in the lithium chloride group were given lithium chloride (140 mg/kg) by gavage, 1 time per day for 8 weeks.Rats in the model group and the blank group were given equal amount of normal saline.Rats were sacrificed and HE staining was performed on the right femoral head.Histopathological changes and necrosis of the femoral head were observed.The left femoral head was used for micro CT examination, and each bone mineral density (BMD), bone volume fraction (BV/TV), trabecular space (Tb.Sp), trabecular bone number (Tb.N), trabecular bone thickness (Tb.Th) were recorded.Results HE staining showed that the trabecular bone in the blank group was complete and regularly arranged, and the bone lacunae were filled with bone cells;the trabecular bone in the model group was disorder, narrow, and fractured, and the marked necrosis of bone widely existed in the empty bone lacunae;the trabecular bone shape in the lithium chloride group was better without obvious stenosis or fracture.However, there were empty bone lacuna in the lithium chloride group which was less than that in the model group.In the blank control group, model group and lithium chloride group, 0, 8 (80%) and 3 (30%) rats with femoral head necrosis were found, respectively (all P<0.05).Compared with the blank control group, the Tb.N and Tb.Th of the model group and the lithium chloride group were all decreased, Tb.Sp was increased, but these in the model group expressed more significantly (all P<0.05), the BMD and BV/TV in the model group was lower than that of the the blank control group and lithium chloride group (all P<0.05).Conclusion Lithium chloride can increase the bone mass of S-ONFH rat femoral head and improve its microstructure, and it has a protective effect on the femoral head.关键词
激素性股骨头坏死/氯化锂/骨量/骨微观结构/大鼠Key words
steroid-induced osteonecrosis of the femoral head (S-ONFH)/lithium chloride/bone mass/bone microstructure/rats分类
医药卫生引用本文复制引用
李云矗,孙平,徐刚,徐成福..氯化锂对激素性股骨头坏死大鼠股骨头微观结构的影响[J].山东医药,2017,57(16):16-19,4.基金项目
牡丹江医学院研究生创新科研项目(2014YJSCX-12) （2014YJSCX-12）
牡丹江医学院科学技术研究项目(ZSSD201623). （ZSSD201623）
