山东医药2017，Vol.57Issue(16)：20-23,4.DOI:10.3969/j.issn.1002-266X.2017.16.006
2型糖尿病患者肠道菌群变化及意义
Variation of intestinal flora in patients with type 2 diabetes mellitus and the significance
摘要
Abstract
Objective To detect the variation of intestinal flora in patients with type 2 diabetes mellitus (T2DM) and the clinical significance.Methods The fecal samples were collected from 8 T2DM patients and 10 healthy participants.Total DNA was extracted from stool samples and submitted to high-throughput sequencing with primers targeting V4 region of the 16s rRNA gene.High-throughput sequencing was used to analyze the abundance and diversity of fecal microbiota.The difference of intestinal flora in patients with different gender, and the relationship between the dominant bacteria in the T2DM patients and fasting plasma glucose (FPG), 1 hour postprandial blood glucose (1h PG) and 2 hour postprandial blood glucose (2h PG) were investigated.Results The Shannon diversity index of T2DM patients and healthy subjects was 3.70±0.29 and 3.30±0.20, respectively, and there was no significant difference between the two groups (P>0.05).The dominant bacteria in the two groups were Firmicutes, Bacteroides, Proteobacteria, Actinobacteria at phylum level, and there was no significant difference in the abundance between the two groups (all P>0.05).In addition, the dominant bacteria were Bacteroides and Lachnospiraceae in the two groups at genus level.Compared with healthy subjects, the abundance of Roseburia was significantly reduced in the T2DM patients (P<0.05).The abundance of the Bacteroides in the female T2DM patients was much higher than that of the male patients, while the abundance of Peptostreptococcaceae was much lower than that of the male patients (P<0.05).The abundance of Faecalibacterium was positively correlated with 2 h PG (r=0.75, P<0.05), and the other dominant bacteria were not correlated with FPG, 1 h PG, 2 h PG (all P>0.05).Conclusion T2DM patients show intestinal flora imbalance with gender differences, and the abundance of Faecalibacterium in T2DM patients is related to blood glucose, which may provide a new therapeutic approach for T2DM.关键词
2型糖尿病/肠道菌群/肠道优势菌属/丰度/血糖/高通量测序Key words
type 2 diabetes mellitus/intestinal flora/intestinal dominant fungi/abundance/blood glucose/high-throughput sequencing分类
医药卫生引用本文复制引用
马苏娴,张锐锐,王苏,邓家良,杨禄,袁国跃..2型糖尿病患者肠道菌群变化及意义[J].山东医药,2017,57(16):20-23,4.基金项目
国家自然科学基金面上项目(81570721、81370965) （81570721、81370965）
江苏省自然科学基金资助项目(BK20151331) （BK20151331）
"六大人才高峰"第十二批高层次人才项目(2015-WSN-006) （2015-WSN-006）
镇江市科技支撑-社会发展重点项目(SH2015028). （SH2015028）
