山东医药2017，Vol.57Issue(16)：24-27,4.DOI:10.3969/j.issn.1002-266X.2017.16.007
miR-449a对食管鳞癌细胞Eca-109增殖、迁移、侵袭的影响及机制
Effects of miR-449a on proliferation, migration and invasion of esophageal squamous-cell carcinoma cell line Eca-109
摘要
Abstract
Objective To investigate the effects of miR-449a on cell proliferation, migration and invasion of esophageal squamous-cell carcinoma cell line Eca-109 and its mechanism.Methods Eca-109 cells were divided into the miR-449a group and the negative control (NC) group, in which miR-449a mimics and NC-mimics were transfected into Eca-109 cells, respectively.Real-time fluorescent quantitative PCR was performed to detect the relative expression of miR-449a in Eca-109 cells.When the expression of miR-449a in Eca-109 cells of the miR-449a group was higher than that of the NC group, indicating Eca-109 cell models with up-regulated miR-449a expression were successfully established.The cell proliferation abilities of the two groups were analyzed by CCK-8 and colony formation assay, which were shown in OD values and cell survival numbers at 12, 24 and 48 h.The cell migration and invasion was measured by Scratch test and Transwell invasion testing system, respectively.Additionally, flow cytometry was performed to detect cell cycle.Real-time fluorescent quantitative PCR and Western blotting were performed to detect the expression of E2F3 mRNA and protein in the two groups.Results At 12, 24 and 48 h, the OD value, the number of survival cells, distance between cells and the number of cells invading into the lower chamber of the miR-449a group were lower than those in the NC group (P<0.05 or P<0.01).The percentage of cells in G1 phase of the miR-449a group was higher than that in the NC group, and the percentage of cells in S phase was lower than that of the NC group (all P<0.05).However, there was no significant difference in the percentage of cells in G2/M phase between the two groups (P>0.05).The relative expression of E2F3 mRNA and protein in the miR-449a group was lower than that in the NC group (all P<0.01).Conclusion miR-449a can inhibit the proliferation, migration and invasion of esophageal squamous-cell carcinoma cell line Eca-109 by inhibiting the expression of target gene E2F3.关键词
食管鳞癌/微小RNA-449a/E2F3基因/细胞增殖/细胞迁移/细胞侵袭Key words
esophageal squamous-cell carcinoma/microRNA-449a/E2F3 gene/cell proliferation/cell migration/cell invasion分类
医药卫生引用本文复制引用
王强强,王梦洁,张玉虹,李皓,高飞,刘阳晨..miR-449a对食管鳞癌细胞Eca-109增殖、迁移、侵袭的影响及机制[J].山东医药,2017,57(16):24-27,4.基金项目
江苏省"333工程"科研项目(BRA2015225). （BRA2015225）
