实验动物与比较医学2017，Vol.37Issue(2)：83-88,6.DOI:10.3969/j.issn.1674-5817.2017.02.001
槲皮素对大鼠非酒精性脂肪肝炎的治疗作用及其机制
Therapeutic Effects and Mechanisms of Quercetin on Non-alcoholic Steatohepatitis in Rats
摘要
Abstract
Objective To investigate the therapeutic effects of quercetin on non-alcoholic steatohepatitis (NASH) in rats,and the effects of quercetin on expressions of nuclear factor-κB (NF-κB).Methods The rats were fed with a high-fat diet for 6 weeks to induce NASH.The NASH model rats were given by gavage with quercetin 40-160 mg/kg for 4 weeks.The levels of serum total cholesterol (TC),triglycer ides (TG),low density lipoprotein cholesterol (LDL-C),high density lipoprotein cholesterol (HDL-C),alanine aminotransferase (ALT),and aspartate aminotransferase (AST) were measured by the automatic blood chemical analyzer.The protein expressions of NF-κB in the hepatic tissues were detected by western blotting while tumor necrosis factor-α (TNF-α),interleukin-6 (IL-6) and interleukin-1 β (IL-1 β) mRNA by RT-PCR.Results Compared with control groups,the serum levels of LDL-C,TC and AST were decreased significantly in the quercetin-treated groups.Oral administration of quercetin improved hepatic steatosis and inflammatory cell infiltration effectively in NASH rats,reduced the mRNA expressions of TNF-α,IL-6 and IL-1β,and down-regulated the protein expression of NF-κB,with a dose-dependent trend which can be observed.Conclusion Quercetin had significant therapeutic benefits to prevent NASH through inhibiting hepatic lipid accumulation,that may be works through regulating NF-κB pathway.关键词
槲皮素/非酒精性脂肪肝炎(NASH)/NF-κB/炎症反应/高血脂Key words
Quercetin/Non-alcoholic steatohepatitis (NASH)/Nuclear factor-κB (NF-κB)/Inflammation/Hyperlipoidemia分类
生物科学引用本文复制引用
李剑波,余陈欢,王志远,方杰,应华忠..槲皮素对大鼠非酒精性脂肪肝炎的治疗作用及其机制[J].实验动物与比较医学,2017,37(2):83-88,6.基金项目
国家自然科学基金面上项目(81573591),浙江省自然科学基金重点项目(LZ17H280001),浙江省省属科研院所专项计划项目(2014F10033) （81573591）
