实验动物与比较医学2017，Vol.37Issue(2)：89-93,5.DOI:10.3969/j.issn.1674-5817.2017.02.002
Afp-cre-lacZ转基因小鼠的构建
Construction of Afp-cre-lacZ Transgenic Mouse Model
摘要
Abstract
Objective To study the function of alpha-fetoprotein (Afp) positive cells during tissues growth and repair or in tumorigenesis,Afp-cre-lacZ linage tracing mouse model was established.Methods Afp-CreERT mice were established by microinjection and identified by real time PCR.Afp-CreERT mice were crossed with Rosa26-1acZ mice.Cre/lacZ both positive mice were kept as Afp-cre-lacZ mice.Results Fifty-six transgenic mice were born after microinjection.Four of them genotyped Cre positive were kept as founder.Each founder was established a strain.Cre expression of each strain was identified via real time PCR.Thus Afp-CreERT transgenic mouse was established.The Cre/lacZ both positive offspring of Afp-CreERT mice were crossed with Rosa26-1acZ mice were kept as Afp-cre-lacZ mice.Identified by real time PCR,X-gal staining and immunofluorescence,Afp-cre-lacZ mice were observed that they could be used in lineage tracing and further studies.Conclusions The Afp-cre-lacZ mice established may be used as a tool for lineage tracing studies.关键词
甲胎蛋白(Afp)/转基因小鼠模型/细胞示踪Key words
Alpha-fetoprotein (Afp)/Transgenic mouse model/Lineage tracing分类
生物科学引用本文复制引用
顾晓雯,孙瑞林,费俭..Afp-cre-lacZ转基因小鼠的构建[J].实验动物与比较医学,2017,37(2):89-93,5.基金项目
国家重点基础研究发展计划(2010CB945501) （2010CB945501）
