实验动物与比较医学2017，Vol.37Issue(2)：108-112,5.DOI:10.3969/j.issn.1674-5817.2017.02.005
吲哚胺-2,3-双加氧酶抑制剂联合铝佐剂对甲型肝炎减毒活疫苗诱导小鼠体液免疫应答的效应分析
Effects of Indoleamine-2,3-dioxygenase Inhibitor Combined with Aluminum Adjuvant on Humoral Immune Response Induced by Hepatitis A Virus Attenuated Live Vaccine in Mice
摘要
Abstract
Objective To investigate the compound effect of indoleamine-2,3-dioxygenase (IDO) inhibitor INCB024360 analogue combined with aluminum adjuvant on humoral immune response induced by hepatitis A virus (HAV) attenuated live vaccine(HepA-l) in mice.Methods The ICR mice were injected respectively with HepA-1 (18EU) and Al(OH)3 (300 μg) and three different doses (150 μg,100 μg,50 μg) of INCB024360 analogue as experimental group,200 μL for each and one immunization.At the same time,set up the blank group,pure vaccine group,aluminum adjuvant control group and single INCB024360 analogue (100 μg) group.The sera from tail vein blood of mice were collected at the end of 4,8,12 and 16 weeks after immunization and the specific IgG antibody against HAV were detected by indirect ELISA method.Results In addition to the blank group,mice in various group generated anti-HAV IgG at 4,8,12,16 weeks after the immunization.The antibody levels increased as time went on and reached peak at 12 weeks,then decreased gradually.The antibody level of compound adjuvant 2 group is the highest.At 8,12 weeks,the antibody level was significantly higher than pure vaccine group,aluminum adjuvant control group and single INCB024360 analogue group (P＜0.05).At 4,8,12,16 weeks,the difference between the single adjuvant group and the simple vaccine group was not statistically significant.Conclusions The compound adjuvant of IDO inhibitor INCB024360 ana logue combined with aluminum adjuvant can obviously enhance the HepA-1 induced humoral immune response in mice,and the immunity enhancement effect is better than aluminum adjuvant control group.But the single INCB024360 analogue does not have significant adjuvant effect.关键词
吲哚胺-2,3-双加氧酶(IDO)/INCB024360类似物/疫苗佐剂/甲型肝炎减毒活疫苗(HepA-1)/体液免疫/佐剂效应Key words
Indoleamine-2,3-dioxygenase (IDO)/INCB024360 analogue/Vaccine adjuvant/Hepatitis A virus attenuated live vaccine (HepA-1)/Humoral immunity/Adjuvant effect分类
生物科学引用本文复制引用
何慧,李彦涵,李建芳,马静,胡云章,胡凝珠..吲哚胺-2,3-双加氧酶抑制剂联合铝佐剂对甲型肝炎减毒活疫苗诱导小鼠体液免疫应答的效应分析[J].实验动物与比较医学,2017,37(2):108-112,5.基金项目
国家科技支撑计划项目(2014BAI01B01) （2014BAI01B01）
云南省创新团队“中国医学科学院医学生物学研究所新型疫苗佐剂应用研究省创新团队”(2011CI140) （2011CI140）
国家自然科学基金项目(31500748) （31500748）
国家重点研究计划生物安全关键技术研究发重点专项(2016YFC1202300) （2016YFC1202300）
