实验动物与比较医学2017，Vol.37Issue(2)：113-117,5.DOI:10.3969/j.issn.1674-5817.2017.02.006
树鼩经甲型肝炎减毒活疫苗、乙型肝炎疫苗及联合硫酸乙酰肝素佐剂免疫后的免疫效果评价
The Immunity Effect Evluation on Tree Shrew by Hepatitis A Virus Attenuated Live Vaccine, Hepatitis B Vaccine Independently or Combined Adjuvant Heparan Sulfate
摘要
Abstract
Objective To investigate the humoral immune response of tree shrew after the immunization of Hepatitis A attenuated live vaccine (HepA-1)、Hepatitis B vaccine (Hbv) independently and combined with adjuvant heparan sulfate (HS).Methods The tree shrews were randomly divided into 10 groups by the type of vaccine,adjuvant and schedules.The specific IgG levels in sera of tree shrew were determined by ELISA at 4,8,12,16 and 20 weeks after the last immunization respectively.Results Except the blank control group,the anti-hepatitis A virus (HAV) and anti-hepatitis B virus (HBV) antibody was detected in all the experimental group at 4 weeks after last immunization,the level of immune antibody were gradually increased with time and reached the peak at the 12 weeks,then decreased gradually.At the same time,the humoral immunity effect of HS adjuvant group were better than that of vaccine group.There is a significant difference on immunity among tree shrews with different antigens and adjuvant by different immunization schedules.Conclusion The immune effect of vaccine immunogenicity and immune enhancing effect on tree shrew confirms the feasibility of the tree shrew as an animal model.关键词
树鼩/甲型肝炎减毒活疫苗(HepA-1)/乙型肝炎疫苗(Hbv)/硫酸乙酰肝素(HS)/体液免疫Key words
Tree shrew/Hepatitis A virus attenuated live vaccine (HepA-1)/Hepatitis B vaccine (Hbv)/Heparan sulfate (HS)/Humoral immunity分类
生物科学引用本文复制引用
王东宝,胡云章,胡凝珠,罕园园,匡德宣,李彦涵..树鼩经甲型肝炎减毒活疫苗、乙型肝炎疫苗及联合硫酸乙酰肝素佐剂免疫后的免疫效果评价[J].实验动物与比较医学,2017,37(2):113-117,5.基金项目
国家科技支撑计划项目(2014BAI01B01) （2014BAI01B01）
国家重点研究计划生物安全关键技术研究发重点专项(2016YFC1202300) （2016YFC1202300）
