实验动物与比较医学2017，Vol.37Issue(2)：118-122,5.DOI:10.3969/j.issn.1674-5817.2017.02.007
自发性糖尿病长爪沙鼠环氧化酶(COX)-2在三种组织中的表达
Analysis on Cyclooxygenase 2 Expression in Different Tissues from Spontaneous Diabetic Mongolian Gerbils
摘要
Abstract
Objective To analyze Cyclooxygenase 2(COX-2) expression level in different tissues of diabetic gerbils and to explore the relationship of diabetes occurrence and development in Mongolian gerbils.Methods Different tissues including skeletal muscle,liver and kidney were collected from 6 diabetic gerbils and 6 control animals respectively.COX-2 mRNA and protein expression level in different tissues were separately detected by Real-time PCR and Western blotting.Results The Real-time PCR showed that compared with control animal,the mRNA expression of COX-2 in skeletal muscle had no obvious difference whereas it exhibited uptrend in liver and kidney.As the results of Western blotting,the difference of COX-2 in skeletal muscle was almost undetectable in diabetes group.The relative expression level in diabetic group exhibited uptrend compared with control in liver and kidney.And there was statistical significance in the kidney.Conclusions COX-2 was high expressed in liver and more remarkably in kidney of diabetic gerbils.It may illustrate that the effect of COX-2 mainly occurred in liver and kidney of diabetic gerbils.关键词
长爪沙鼠/Ⅱ型糖尿病/COX-2/实时PCR/Western blottingKey words
Mongolian gerbils/Type 2 diabetes/Cyclooxygenase 2 (cox-2)/Real-time PCR/Western blotting分类
生物科学引用本文复制引用
王菲菲,吕建祎,龚菁菁,霍学云,路静,郭萌,刘欣,李长龙,杜小燕,陈振文..自发性糖尿病长爪沙鼠环氧化酶(COX)-2在三种组织中的表达[J].实验动物与比较医学,2017,37(2):118-122,5.基金项目
国家科技支撑计划(No.2015BAI09B01) （No.2015BAI09B01）
国家自然科学基金(Nos.31272393 and 31402027) （Nos.31272393 and 31402027）
北京市自然科学基金(No.7141002) （No.7141002）
