生物技术通报2017，Vol.33Issue(5)：94-101,8.DOI:10.13560/j.cnki.biotech.bull.1985.2017.05.014
水稻OsRhoGAP1基因的序列特征及表达特性分析
Sequence Characterization and Expression Analysis of OsRhoGAP1 Gene in Rice
摘要
Abstract
A function-unknown gene was isolated from a cDNA library of young spike of rice and designated as OsRhoGAP1. Based on the bioinformatics and modern molecular biology,we systematically surveyed its expression patterns at different development stages and under different hormones treatments. Bioinformatics analysis showed that OsRhoGAP1 had typical structural feature of plant RhoGAP protein,including CRIB motif and RhoGAP domain. qRT-PCR analysis revealed that OsRhoGAP1 gene presented specifically spatio-temporal characteristics,i.e.,relative high expression levels in root and leave at the seedling stage,in young panicle and leave at the panicle stage, and in root at the mature stage. Furthermore,the transcription of OsRhoGAP1 was strongly induced by ABA,SA,GA,and MeJA,but suppressed by IAA and 6-BA. It was speculated that the expression of OsRhoGAP1 was regulated by hormones.关键词
水稻/OsRhoGAP1基因/序列分析/表达分析/植物激素Key words
rice/OsRhoGAP1gene/sequence analysis/expression analysis/plant hormones引用本文复制引用
闫鑫甜,黄俊骏,楼晨,葛慧雯,梁卫红..水稻OsRhoGAP1基因的序列特征及表达特性分析[J].生物技术通报,2017,33(5):94-101,8.基金项目
国家自然科学基金项目(31171182),河南省高校科技创新团队支持计划(15IRTSTHN020) （31171182）
