生物技术通报2017，Vol.33Issue(5)：108-116,9.DOI:10.13560/j.cnki.biotech.bull.1985.2017.05.016
蒙古沙冬青AmMYB4-like基因的克隆与表达分析
Cloning and Expression Analysis of AmMYB4-like in Ammopiptanthus mongolicus
摘要
Abstract
Based on our previous work,a gene encoding MYB transcription factor was cloned from Ammopiptanthus mongolicus,and it was designated as AmMYB4-like. The full length of the AmMYB4-like cDNA was 1,145 bp,including a 960 bp opening reading frame(ORF) which encoded a 319-amino acid peptide. AmMYB4-like contained R2 and R3 conserved domains and belonged to the R2R3-MYB subfamily. Quantitative real-time PCR(qRT-PCR)analyses revealed that AmMYB4-like was up-regulated in leaves under low temperature and drought stresses,but it was mainly induced by drought stress in roots. Plant expression vector pPZP212- AmMYB4-like was constructed for further research of AmMYB4-like.关键词
蒙古沙冬青/AmMYB4-like/基因克隆/表达分析/植物表达载体构建Key words
Ammopiptanthus mongolicus/AmMYB4-lik/gene cloning/expression analysis/construction of plant expression vector引用本文复制引用
肖自华,高飞,孙会改,周宜君..蒙古沙冬青AmMYB4-like基因的克隆与表达分析[J].生物技术通报,2017,33(5):108-116,9.基金项目
国家自然科学基金项目(31370356,31670335),建设世界一流大学(学科)和特色发展引导专项资金(YDZXXK201619, 2015MDTD08C) （31370356,31670335）
