生物技术通报2017，Vol.33Issue(5)：183-189,7.DOI:10.13560/j.cnki.biotech.bull.1985.2016-1098
一种新型蛋白酶的酶学特性及脱毛应用
Enzymatic Characteristics of a Novel Protease and Its Application for Dehairing
摘要
Abstract
Protease can be applied in dehairing process of leather industry as an eco-friendly product. We aim to clear the dehairing performance and the process of enzymatic dehairing of JW-3 protease produced by recombinant strain WB800N/pHT43-npr,and to provide technical support for clean technology of leather manufacture. The thermostability and pH value tolerance of JW-3,as well as the effects of metal ions and chemicals on its enzyme activity were investigated. Meanwhile,the ability of enzymatic hydrolysis on collagen,and the comparison between enzymatic dehairing and lime-sulfide dehairing process on cowhide were evaluated. Results showed that JW-3 protease was in poor thermal resistance that enzyme activity began to decay after remaining 1 h at 40℃,and presented solid stability at pH 6 and 7,and was stable in the surfactant of Triton X-100,Tween20,Tween80 ;moreover,metal ions of Ca2+,Mn2+ activated its activity. Furthermore, JW-3 protease was incapable of hydrolyzing collagen,and showed the equivalent dehairing performance with lime-sulfide process,also by which nondestructive depilated hair could be recycled. Thus,JW-3 protease can complete cowhide hair removal process,but not hydrolyze the collagen of cowhide,and can be used as an environmentally friendly alternative to the conventional chemical process.关键词
制革业/清洁化技术/蛋白酶/酶学性质Key words
leather manufacture/clean-technology/protease/enzymatic properties引用本文复制引用
郑翔,杨何宝,胡美荣,刘春卯,吴芳彤,曹倩荣..一种新型蛋白酶的酶学特性及脱毛应用[J].生物技术通报,2017,33(5):183-189,7.基金项目
河北省省级省校科技合作开发资金项目(2016049819) （2016049819）
