生物技术通报2017，Vol.33Issue(5)：219-226,8.DOI:10.13560/j.cnki.biotech.bull.1985.2016-0968
鰤鱼诺卡氏菌DmpA基因的克隆及亚细胞定位研究
Gene Cloning and Subcellular Localization of DmpA from Nocardia seriolae
摘要
Abstract
Nocardia seriolae,a facultative intracellular bacterium,is the main pathogen of fish nocardiosis. Bioinformatics analysis showed that the DmpA gene of N. seriolae encoded a secreted protein,and may be co-located with mitochondria of the host cell. In this study, the recombinant plasmids pEGFP-DmpA and pcDNA-DmpA were constructed,extracellular product of N. seriolae was identified,and the subcellular localization and over-expression of DmpA were carried out. The results showed that the protein DmpA was identified in the extracellular products of N. seriolae,and confirmed as a secreted protein. Subcellular localization of DmpA-GFP fusion proteins were evenly distributed in the whole cell of FHM cells,and were not coincided with the distribution of mitochondria,indicating that the protein DmpA was not co-localized with the mitochondria. The expression of DmpA changed the distribution of mitochondria into lumps in the FHM cell. The over-expression of DmpA in FHM cells had no significant effect on the nucleus,revealing that the gene had no function on inducing cell apoptosis. The cloning,subcellular localization and over-expression of DmpA from N. seriolae laid the foundation for further studying the function of the gene and promoting the understanding of the pathogenic mechanism of N. seriolae.关键词
鰤鱼诺卡氏菌/DmpA基因/分泌蛋白/亚细胞定位Key words
Nocardia seriolae/gene DmpA/secreted protein/subcellular localization引用本文复制引用
夏立群,陈锐敏,廖保山,徐亮,苏泽杰,童邦卓..鰤鱼诺卡氏菌DmpA基因的克隆及亚细胞定位研究[J].生物技术通报,2017,33(5):219-226,8.基金项目
广东省科技发展专项资金项目(2016A050502061),广东省自然科学基金(2014A030313602),深圳大鹏新区产业发展专项资金项目(KY20160207),广东省攀登计划项目(pdjh2016b0234) （2016A050502061）
