山西农业大学学报（自然科学版）2017，Vol.37Issue(2)：121-125,5.
碱性蛋白酶和复合蛋白酶双酶水解羊骨粉的工艺研究
The Study on the Hydrolysis of Alcalase and Protamex to Sheep Bone Powder
摘要
Abstract
[Objective]Alcalase and protamex were the best enzymes for collagen polypeptide that hydrolyzed from the sheep bone through screening.[Methods]This research compared the effect of sequentially and simultaneously hydrolyzing to further improve the enzyme effect and to determine the best condition for hydrolyzing.[Results]This experiment showed that:The group of simultaneously hydrolyzing AB2 that the hydrolysis of indicators were extremely higher than the single (P＜0.05) compared with sequentially hydrolyzing is insignificant(P＞0.05) but saved time extremely in condition of 9％ substrate,5％ (E/S) alcalase and 4.25％ (E/S) protamex,initial pH 8.5,50 ℃,5 h.From researching that the degree of hydrolysis,amount of amino-acid nitrogen,amount of formed polypeptides,the obtaining rate of oligopeptides partly researched to 29.79％,28.13％,110.43 mg·g-1 and 73.88％.[Conclusion]To sum up,double enzyme simultaneously hydrolyzing not only could improve the enzyme effect but also could provide experimental support for the preparation of collagen polypeptide from sheep bone.关键词
碱性蛋白酶/复合蛋白酶/羊骨粉/胶原多肽/双酶水解Key words
Alcalase/Protamex/Sheep bone/Collagen peptide/Double enzyme hydrolysis分类
轻工纺织引用本文复制引用
张琪,甄守燕,霍乃蕊..碱性蛋白酶和复合蛋白酶双酶水解羊骨粉的工艺研究[J].山西农业大学学报（自然科学版）,2017,37(2):121-125,5.基金项目
国家自然科学基金(31201347) （31201347）
山西省回国留学人员项目(2014-042) （2014-042）
