生殖医学杂志2017，Vol.26Issue(5)：409-414,6.DOI:10.3969/j.issn.1004-3845.2017.05.004
PCOS合并代谢综合征患者卵泡液激肽谱与卵子质量的关系
Cytokine profile of follicular fluid and oocyte quality of PCOS patients with metabolic syndrome
摘要
Abstract
Objective:To provide insights into the correlation between lipid metabolic pattern and cytokine profile in follicular fluid (FF) and embryo quality in women with polycystic ovary syndrome (PCOS) & metabolic syndrome (MS).Methods:Ninety women undergoing IVF treatment,including 60 PCOS patients and 30 age-matched controls,were recruited in this prospective cohort study.The PCOS patients were divided into PCOS non MS (n=30) and PCOS & MS (n=30) groups.All patients received controlled ovarian stimulation by using GnRH antagonist protocol.The follicular fluid of single dominant follicle with diameter 18-21 mm was collected on the day of oocyte retrieval.The cytokine expression in follicular fluid was detected by suspension array technique (SAT).The lipid metabolism,follicle expression and related embryonic quality in follicular fluid were compared among the groups.Results:The basic hormone levels of LH,E2,progesterone on HCG triggering day and the levels of LH,E2,progesterone on the day of oocyte retrieval,and the follicular diameter were not statistically different (P>0.05).PCOS & MS group had significantly increased BMI,TG and decreased serum HDL concentrations in serum and FF compared with the other two groups(P<0.05).The PCOS & MS group was associated with significantly higher TC & TG and lower HDL concentrations in FF compared with the control group (P<0.05).The levels of cholesterol and TNF-α in follicular fluid of PCOS patients with metabolic syndrome were significantly higher than those of the other two groups (P<0.05),while G-CSF level was significantly decreased (P<0.05).There were no significant differences in the other 15 cytokine levels (P>0.05).The G-CSF level in FF was negatively correlated with TG (R2=0.29) and TC (R2=0.25),while TNF-α concentration was positively associated with TG (R2=0.26).The percentage of the high-quality embryo significantly decreased in the PCOS & MS group (20.0%) than in the PCOS group (38.4%) and control group (34.6%)(P<0.05).Conclusions:The abnormal indexes of lipid metabolism in follicular fluid of patients with PCOS complicated with metabolic syndrome were mainly related to G-CSF and TNF-α in the cytokine expression profile.Since the kinin system has a direct effect on oocyte development,the reasons for the decline in oocyte development potential in PCOS patients with metabolic syndrome are likely to involve this mechanism.关键词
多囊卵巢综合征/代谢综合征/激肽/卵母细胞Key words
PCOS/Metabolic syndrome/Cytokine/Oocyte引用本文复制引用
牛志宏,叶瑶,夏兰,冯云..PCOS合并代谢综合征患者卵泡液激肽谱与卵子质量的关系[J].生殖医学杂志,2017,26(5):409-414,6.基金项目
上海市自然科学基金(16ZR1421500) （16ZR1421500）
