天然产物研究与开发2017，Vol.29Issue(3)：382-386,5.DOI:10.16333/j.1001-6880.2017.3.003
ERK1/2信号通路介导大蒜素对β-分泌酶亚基Bace1的调控作用
Regulation Effect of Allicin on Beta Secretase Subunit Bace1 Mediated by ERK1/2 Signaling Pathway
张浩 1王玥 2潘海1
作者信息
- 1. 沈阳医学院附属中心医院第二神经外科,沈阳110024
- 2. 辽宁中医药大学基础医学院,沈阳110847
- 折叠
摘要
Abstract
In this study,the regulation effect of ERK1/2 signal pathway mediated allicin on beta secretase subunit Bace1 was investigated by immunofluorescence,Western blot,Real-Time PCR and ELISA methods.It was found that the Bace1 expression in SH-SY5Y cells which treated with allicin was lower than control group,but treated with allicin and ERK1/ 2 pathway inhibitors PD98059,the expression of Bace1 was rose.The results showed that allicin can down-regulate Bace1 expression through ERK1/2 pathway.ELISA results showed that the expression of A beta 1-40 and A beta 1-42 were decreased in SH-SY5Y cells which treated with allicin,while the expressions were increased in SH-SY5Y cells which treated with allicin and PD98059,compared with allicin treatment group.These results prompted that allicin can inhibit the beta secretase activity,downregulate Bacel expression,and suggested that allicin had protective effect on neurons by decreasing the production of Aβ.关键词
大蒜素/SH-SY5Y/β-分泌酶/Bace1/ERK1/2信号通路Key words
allicin/SH-SY5Y/beta secretase/Bace1/ERK1/2 signal pathway分类
医药卫生引用本文复制引用
张浩,王玥,潘海..ERK1/2信号通路介导大蒜素对β-分泌酶亚基Bace1的调控作用[J].天然产物研究与开发,2017,29(3):382-386,5.
