天然产物研究与开发2017，Vol.29Issue(3)：387-392,6.DOI:10.16333/j.1001-6880.2017.3.004
藁本内酯神经炎症抑制作用与PPARγ依赖的TLR4/NF-κB信号通路的相关性
Z-Ligustilide Inhibits LPS-induced Neuroimflammation via PPARγ-dependent Suppression of TLR4/NF-κB Signaling Pathway
摘要
Abstract
Our previous studies reported the anti-neuroinflamatory and neuroprotective effects of Z-ligustilide (LIG).The interaction between peroxisome proliferator-activated receptor γ(PPARγ) and TLR4/NF-κB signaling is associated with neuroinflammatory response and inflammatory injury in brain.The present study is to investigate whether PPARγ-modulating TLR4/NF-κB signaling pathway is implicated in the molecular mechanism of LIG anti-neuroinflammation.Male rats were pretreated with vehicle,LIG,or GW9662 (selective PPARγ antagonist) plus LIG,followed by the intracerebroventricular injection of lipopolysaccharide(LPS,a TLR4 ligand).The results showed that LIG does-dependently reduced LPS-induced increase in the concentrations of pro-inflammatory mediators (tumor necrosis factor o,monocyte chemoattractant protein-1),TLR4 protein expression in rat cerebral cortex.Moreover,LIG significantly prevented LPS-induced alterations in the transcription activities of NF-κB and PPARγ in the rat cortex.Remarkably,the anti-neuroinflammatory effects of LIG were effectively attenuated by GW9662,as indicated by an obvious increase in production of pro-inflammatory mediators and activation of TLR4/NF-κB signaling pathway following co-treatment with LIG and GW9662.Taken together,these findings suggested that LIG may prevent neuroinflammatory response through TLR4/NF-κB signaling inhibition in a PPARγ-dependent manner.关键词
藁本内酯/LPS/神经炎症/TLR4/NF-κB/PPARγKey words
LIG/LPS/neuroimflammation/TLR4/NF-κB/PPARγ分类
医药卫生引用本文复制引用
史梦琪,旷喜,刘晓娇,王良芬,杜俊蓉..藁本内酯神经炎症抑制作用与PPARγ依赖的TLR4/NF-κB信号通路的相关性[J].天然产物研究与开发,2017,29(3):387-392,6.基金项目
四川大学研究生课程建设项目(2016KCJ137) （2016KCJ137）
