天然产物研究与开发2017，Vol.29Issue(3)：393-399,7.DOI:10.16333/j.1001-6880.2017.3.005
体内外相结合筛选醋制南五味子抗糖尿病活性部位
Screening of Anti-diabetic Active Fractions from South Schisandra with Vinegar in vivo and in vitro
摘要
Abstract
In this study,the antidiabetic active fraction of Schisandra chinensis was screened in vitro and in vivo using the inhibition rates of α-amylase and α-glucosidase as evaluation index.Mice were intraperitoneally injected with streptozotocin(STZ,140 mg/kg) to develop diabetic animal model;Alanine aminotransferase(GPT),high density l ipoprotein cholesterol (HDL-C),total cholesterol (T-CHO),total triglycerides (TG) in the serum,malondialdehyde (MDA) and superoxide dismutase(SOD) in the liver tissue were measured.The effects of four chemical fractions (Petroleum ether,chloroform,n-butanol,water)were difference.The chemical fraction of petroleum ether from S.chinensisshowed the best inhibition effect on a-amylase;Petroleum ether and chloroform fractions showed the best inhibition effect on o-glucosidase.The in vivo study showed that each administration group can lower blood sugar,improve insulin content,and enhance the mice glucose tolerance,but the effect of petroleum ether and chloroform fractions was obvious.The effects on HDL-C,GOT,GPT,TG and T-CHO were significantly different in the petroleum ether and chloroform fractions.The effect on MDA and SOD contents in the liver tissue of petroleum ether and chloroform fractions were obvious,but there was no obvious change in the other fractions.Both results of in vitro and in vivo pharmacodynamic evaluation showed that the antidiabetic effect of petroleum ether and chloroform chemical fractionswas obvious.关键词
醋制南五味子/化学部位/糖尿病Key words
South Schisandra with vinegar/chemical fraction/diabetes分类
医药卫生引用本文复制引用
邓翀,南美娟,张化为,姜祎..体内外相结合筛选醋制南五味子抗糖尿病活性部位[J].天然产物研究与开发,2017,29(3):393-399,7.基金项目
国家自然科学基金(30902002,81374028) （30902002,81374028）
陕西省中医药管理局项目(13-ZY014) （13-ZY014）
