天然产物研究与开发2017，Vol.29Issue(3)：400-403,4.DOI:10.16333/j.1001-6880.2017.3.006
小单孢菌FIM060152中抗肿瘤活性成分的提取分离纯化
Purification and Identification of Anti-tumor Antibiotic from Micromonospora FIM060152
摘要
Abstract
The purpose of the present study was to isolate,purify and identify antitumor compounds from the fermentation broth of Micromonospora FIM060152.The secondary metabolite was isolated and purified by macroporous resin column chromatography,silica gel chromatography and preparative chromatography;the structure was identified by physicochemical properties,LC-MS and NMR spectral data.The antitumor activity was detected by DNA damage.As a result,compound FIM060152-C1 was isolated from the strain and identified as CalicheamicinγI1.DNA damage exhibited that FIM060152-CL had strong antitumor activity on plasmid pBR322,with MIC value of 0.2 μg/mL.关键词
小单孢菌FIM060152/calicheamicinγI1/分离纯化/DNA断裂活性Key words
Micromonospora FIM060152/calicheamicinγI1/purification/DNA damage分类
化学化工引用本文复制引用
陈丽,林如,王海龙,方志锴,江红,连云阳..小单孢菌FIM060152中抗肿瘤活性成分的提取分离纯化[J].天然产物研究与开发,2017,29(3):400-403,4.基金项目
福建省自然科学基金(2014J01275) （2014J01275）
公益类科研院所专项(2014R1009-4) （2014R1009-4）
福州市生物制药行业技术创新中心(2016-PT-36) （2016-PT-36）
福建省化药技术重大研发平台(2014Y200) （2014Y200）
阅海洋高新项目(2016-25) （2016-25）
