天然产物研究与开发2017，Vol.29Issue(3)：404-409,476,7.DOI:10.16333/j.1001-6880.2017.3.007
链霉属放线菌Streptomyces antibioticus H12-15发酵物的化学成分研究
Chemical Compounds from the Fermented Broth of Actinomycete Strain Streptomyces antibioticus H 12-15
摘要
Abstract
By using various sorts of column chromatography,ten compounds were isolated from the bioactive fraction of Streptomyces antibioticus strain H12-15 which derived from the sea bed mud at the mangrove district of South China Sea.The structures of these compounds were identified as actinomycin X2 (1),actinomycin D(2),lanosterol (3),4,4-dimethylzymosterol(4),ergosterol peroxide(5),24-methyl-5α-cholesta-7,22-diene-3β,6α-diol(6),3β,5α,9α-trihydroxy ergosta-7,22-diene-6-one (7),3β,5 α,9α,14α-tetrahydroxyergosta-7,22-diene-6-one (8),5α,6α-epoxy-ergosta-8,14,22-triene-3β,7α-diol (9),β-sitosterol(10) based on the spectroscopic analysis.Among them,compounds 3-10 were isolated from Streptomyces species for the first time.Cytotoxicities of these compounds against cancer cell lines were evaluated in vitro by MTY assay.Among them,compounds 1 and 2 showed significant growth inhibition of tumor cells,with IC50 values lower than 1.6 μg/mL,and compound 9 exhibited mild cytotoxic activity.关键词
抗生链霉菌/红树林/次级代谢产物/放线菌素/甾醇Key words
Streptomyces antibioticus/mangrove/metabolites/actinomycin/sterols分类
医药卫生引用本文复制引用
胡辰,陈芳,郑新恒,林壁润,周光雄..链霉属放线菌Streptomyces antibioticus H12-15发酵物的化学成分研究[J].天然产物研究与开发,2017,29(3):404-409,476,7.基金项目
国家自然科学基金面上项目(81373320) （81373320）
广东省自然基金团队项目(2015 A030312002) （2015 A030312002）
暨南大学教育部研究基金(21612114) （21612114）
