天然产物研究与开发2017，Vol.29Issue(3)：425-430,6.DOI:10.16333/j.1001-6880.2017.3.011
麻欠产地对精油化学成分及抗炎效果的影响
Chemical Composition and Anti-Inflammatory Effects of Essential Oil of Maqian(Zanthoxylum myriacanthum var.pubescens Huang) Collected in Three Locations of Xishuangbanna, Yunnan
摘要
Abstract
In order to understand the effects of location on the chemical composition and anti-inflammatory effects of Maqian (Zanthoxylummyriacanthum var.pubescens Huang) essential oil (MQEO),fruits of Maqian were collected from three locations in Xishuangbanna(Menglun,Mengwang,Mengxing).The essential oils were obtained by hydrodistillation.The chemical composition was analyzed using GC-MS and the anti-inflammatory effect was determined by RAW264.7cells.A total of 21 kinds of substances were identified in three MQEOs among which alkenes and alcohols were the dominant components.The chemical composition of MQEOs from Menglun and Mengxing was more similar,whereas MQEO from Mengwang showed marked difference in the content of several major components from those in Menglun and Mengwang samples.RAW264.7 cells were pretreated with vehicle,Dex,or MQEO,and then cultured with LPS for 24 h.NO was determined by Griess reagent.TNF-α was determined by ELISA.Three MQEO all exhibited dose-dependent inhibition of LPS-stimulated production of NO and TNF-α.These results showed that despite difference in the chemical composition of MQEO from three locations,their anti-inflammatory effects remain the same.关键词
麻欠精油/产地差异/化学成分/RAW264.7/抗炎Key words
Maqian essential oil/producing area/chemical composition/RAW264.7/anti-inflammatory分类
医药卫生引用本文复制引用
董丽华,郭娟,张红霞,杜芝芝,许又凯,张萍..麻欠产地对精油化学成分及抗炎效果的影响[J].天然产物研究与开发,2017,29(3):425-430,6.基金项目
中国科学院百人计划(Y3ZK101B01) （Y3ZK101B01）
