天然产物研究与开发2017，Vol.29Issue(3)：435-438,471,5.DOI:10.16333/j.1001-6880.2017.3.013
连翘抗炎药效物质基础筛选研究
Investigation on Anti-inflammatory Components of Forsythia suspensa
摘要
Abstract
In this study,the anti-inflammatory effect of major components of Forsythia suspensa was explored and its bioactive components with anti-inflammatory effect were screened to provide reference for the development of new anti-inflammatory drugs of F.suspensa.Then xylene-induced mice ear swelling models were established and the swelling degree was calculated.The content of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) in mice serum were detected by enzyme-linked immunosorbent assay(ELISA).The swelling degree and the content of TNF-α and IL-6 in mice serum of medication administration groups were compared with those of control group respectively.Compared with the control group,phillyrin had no significant inhibition effect on swelling degree or the production of TNF-α and IL-6,and there was no significant difference between the two groups (P ＞ 0.05).Compared with the control group,significant differences of the swelling degree and the production of TNF-α and IL-6 were detected in phillygenin,forsythiaside A and forsythiaside B group respectively(P ＜ 0.05).Thus it can be seen that by oral administration,only the three selected components of F.suspense,including phillygenin,forsythiaside A and forsythiaside B,had anti-inflammatory effects.By oral administration,the anti-inflammatory effect of phillyrin had not been found yet,but phillygenin,forsythiaside A and forsythiaside B had stronger anti-inflammatory effects.Meanwhile,it can be speculated that the anti-inflammatory mechanism of F.suspensa may be related with its inhibition effect of the production of TNF-α and IL-6 and the effect of multiple components.关键词
连翘苷/连翘脂素/连翘酯苷A/连翘酯苷B/抗炎Key words
phillyrin/phillygenin/forsythiaside A/forsythiaside B/anti-inflammatory effect分类
医药卫生引用本文复制引用
全云云,袁岸,龚小红,彭成,李芸霞..连翘抗炎药效物质基础筛选研究[J].天然产物研究与开发,2017,29(3):435-438,471,5.基金项目
国家自然科学基金面上项目(81373943,81573583) （81373943,81573583）
四川省杰出青年基金(2013JQ0018) （2013JQ0018）
四川省科技厅省青年科技创新研究团队专项(2017TD0007,2015TD0028,2016TD0006) （2017TD0007,2015TD0028,2016TD0006）
成都中医药大学科技发展基金(ZRYB201425) （ZRYB201425）
