天然产物研究与开发2017，Vol.29Issue(3)：472-476,5.DOI:10.16333/j.1001-6880.2017.3.020
雷公藤甲素致小鼠肝损伤的机制研究
Mechanism of Triptolide-induced Liver Injury
摘要
Abstract
In this study,the mechanism of triptolide (TP)-induced liver injury was investigated.A total of 20 female C57BL/6 mice were randomly divided into two groups.Serum alanine aminotransaminase(ALT) levels and hepatic pathological change were used to explore the degree of liver damage,and hepatitic interleukin (IL)-17,IL-6 were detected through RT-PCR and ELISA.The mRNA expression of hepatic retinoid related orphan receptor(ROR-γt) was also examined by RT-PCR.The expression of hepatic Toll-like receptor4 (TLR4) was examined by Western blot.Data showed that TP effectively increased serum ALT levels(P ＜ 0.005,vs control group) and promoted hepatic pathological damage.Meanwhile,TP also significantly promoted the expression of IL-17,IL-6,ROR-γt mRNA and IL-17,IL-6 protein in mouse liver(P ＜ 0.005).Furthermore,the protein expression of TLR4 was obviously increased,compared with control group (P ＜ 0.005).Taken together,the result suggested that TP induced liver injury through activating the TLR4 signal,increasing the expression of IL-17,IL-6 and Th17 specific transcription factor ROR-γt,and promoting the activation of Th17 cells and enhancing the inflammatory function.关键词
雷公藤甲素/肝损伤/炎症因子/C57BL/6小鼠Key words
triptolide/liver injury/cytokines/C57BL/6 mouse分类
医药卫生引用本文复制引用
于淑静,魏彩冰,陶崑,周莲娣..雷公藤甲素致小鼠肝损伤的机制研究[J].天然产物研究与开发,2017,29(3):472-476,5.基金项目
重庆市基础与前沿研究项目(cstc2014jcyjA10108) （cstc2014jcyjA10108）
中央高校基本业务费(CQDXWL-2014-Z007) （CQDXWL-2014-Z007）
中央高校基本业务费(201310611045) （201310611045）
