天然产物研究与开发2017，Vol.29Issue(3)：477-481,5.DOI:10.16333/j.1001-6880.2017.3.021
蜂胶黄酮Pinobanksin-3-acetate对人胃癌SGC-7901细胞中HSPA6、GEM、FOS、RGS2、GADD45G基因表达的影响
Effects of Propolis Flavonoids Pinobanksin-3-acetate on the Expression of HSPA6, GEM, FOS, RGS2 and GADD45G Gene in Human Gastric Cancer Cell Line SGC-7901
摘要
Abstract
In order to investigate the effects of Propolis Flavonoids Pinobanksin-3-acetate(PB3A) on gene expression changes in human gastric cancer cell line SGC-7901,the cellline was treatedwith different concentrations of PB3A (0,40,80 μg/mL),and themRNAexpressionof HSPA6,GEM,FOS,RGS2 andGADD45 G geneswas detected by real-time quantitative PCR.The result showed that after treated the cell line with 40 μg/mL of PB3A,the expression of HSPA6,GEM and FOS genes were significantly increased (P ＜ 0.05).The differential expression rates were 0.12 (HSPA6),2.17 (GEM) and 2.1 (FOS) respectively.However,theexpressionof GADD45Gand RGS2were not significantly increased(P ＞ 0.05)incomparison with untreated control group;After treated the cell linewith 80 μg/mL of PB3A,the expression of HSPA6 gene was significantly (P ＜0.05) increased,the differential expression rate of HSPA6 gene was 0.5,and the expressionofGEM,FOS,RGS2 and GADD45 G geneswere not significantlyincreased (P ＞ 0.05) by comparing to the control group.In conclusion,It was deduced that PB3A affected gastric cancer cell proliferation through regulating the expression of HSPA6,GEM and FOSgenes.关键词
PB3A/胃癌SGC-7901细胞/基因表达Key words
pinobanksin-3-acetate/gastric cancer cell line SGC-7901/gene expression分类
医药卫生引用本文复制引用
阿依努尔·玉苏普,胡尼其古丽·阿巴克,其曼古丽·吐尔洪,吾热娅提·克维尔,依米提·热合慢..蜂胶黄酮Pinobanksin-3-acetate对人胃癌SGC-7901细胞中HSPA6、GEM、FOS、RGS2、GADD45G基因表达的影响[J].天然产物研究与开发,2017,29(3):477-481,5.基金项目
国家自然科学基金(31260280) （31260280）
